Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,667 results for: Tet-on

Clear keywords
Showing: 5181 - 5200 of 7667 results

  2. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bactā€¦
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

    Enlarge

  5. pQCXIP GFP-MOSPD2 W201E

    Plasmid
    #186471
    Purpose
    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Article
    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    W201E (no more binding to lipid droplets)
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186471-plasmid-map-sequence-id-366065

    Enlarge

  7. pCW-ND-Cdt1-HA-Puro

    Plasmid
    #193767
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-HA (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, Ī”Cy mutation (aa68-70 tā€¦
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193767-plasmid-map-sequence-id-380216

    Enlarge

  10. EW489 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8[G2V G4W S6L G7L G10W G11D G13L G17M G19I G22M G23Q S24Q]-FKBP (FLP-IN)

    Plasmid
    #236147
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to FKBP1A, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-FKBP
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236147-plasmid-map-sequence-id-468434

    Enlarge

  11. EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)

    Plasmid
    #236159
    Purpose
    Express NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    NZF-deImmunLink-FRB
    Expression
    Mammalian
    Mutation
    T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236159-plasmid-map-sequence-id-468430

    Enlarge

  12. EW263 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R(FLP-IN)

    Plasmid
    #236126
    Purpose
    Plasmid encoding ADAR2dd attached to PUF-9R under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    ADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    E488Q, T501A in ADAR2dd
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236126-plasmid-map-sequence-id-468414

    Enlarge

  13. EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)

    Plasmid
    #236146
    Purpose
    Plasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    NZF-deImmunLink-FRB
    Expression
    Mammalian
    Mutation
    N2093W, T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236146-plasmid-map-sequence-id-468416

    Enlarge

  17. pENeCFPCNAL2 pc922

    Plasmid
    #167563
    Purpose
    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).
    Depositor
    Cristina Cardoso
    Article
    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.
    Inserts
    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    167563-plasmid-map-sequence-id-403652

    Enlarge

  20. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107578
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKIā€¦
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fbp promoter Bacillus megaterium DSM319
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107578-plasmid-map-sequence-id-209151

    Enlarge

Showing: 5181 - 5200 of 7667 results