We narrowed to 11,635 results for: Vars

1. pCMV-dCas12a-AID

   Plasmid

   #193647

   Purpose

   Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hAID, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-BE

   Plasmid

   #193648

   Purpose

   Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-rAPOBEC1, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas12a-CDA1

   Plasmid

   #193649

   Purpose

   Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-PmCDA1, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCas12a-eAID

   Plasmid

   #193650

   Purpose

   Expression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-eAID, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Talin1(ΔR4-12)-mCherry

   Plasmid

   #212006

   Purpose

   Expression of mouse talin1 (1-913 + 2296-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

   Insert

   Talin1(ΔR4-12)-mCherry (Tln1 Mouse)
   

   Tags

   mCherry fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Talin1(ΔR1-12)-mCherry

   Plasmid

   #212008

   Purpose

   Expression of mouse talin1 (1-490 + 2296-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

   Insert

   Talin1(ΔR1-12)-mCherry (Tln1 Mouse)
   

   Tags

   mCherry fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Talin1(ΔR1-10)-mCherry

   Plasmid

   #212007

   Purpose

   Expression of mouse talin1 (1-490 + 1974-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

   Insert

   Talin1(ΔR1-10)-mCherry (Tln1 Mouse)
   

   Tags

   mCherry fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLINE1mORF1-ΔORF2-scFvFc

   Plasmid

   #206023

   Purpose

   The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

   Plasmid

   #200639

   Purpose

   Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   STIM1 (STIM1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBQ10:LYK5_At-3xHA

    Plasmid

    #202192

    Purpose

    Express Arabidopsis thaliana LYK5 gene under UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    LYSM-CONTAINING RECEPTOR-LIKE KINASE 5 (LYK5 Mustard Weed)
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNMSB96

    Plasmid

    #199316

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

    Plasmid

    #197421

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

    Plasmid

    #197422

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

    Plasmid

    #197423

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

    Plasmid

    #197424

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

    Plasmid

    #197425

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2/shCol1a1/Scarlet_Seq1.2

    Plasmid

    #201404

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Expression

    Mammalian

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2/shCol1a1/Scarlet-Seq1.1

    Plasmid

    #201401

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Expression

    Mammalian

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2/Col1a1/GFP4_Seq 1.1

    Plasmid

    #201400

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Expression

    Mammalian

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-In18runon-PTPN22

    Plasmid

    #195377

    Purpose

    A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Gateway donor vector

    Mutation

    Intron 18-runon PTPN22

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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