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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,942 results for: sequence

Showing: 5221 - 5240 of 12942 results

  4. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  5. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid
    #29752
    Depositor
    Edward McCabe
    Insert
    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    Use
    Tags
    EGFP, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; Reporter is nuclear localized EGFP;…
    Promoter
    Available since
    Oct. 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    29752-plasmid-map-sequence-id-213749

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  6. EYFP-ER-0

    Plasmid
    #56589
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170818-plasmid-map-sequence-id-334731

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  8. pKG96

    Plasmid
    #63072
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-A (CENPA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    A12V, C75Y, and L121F mutations compared to GenBa…
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63072-plasmid-map-sequence-id-134723

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  13. pBJ-FLAG-hPar4

    Plasmid
    #53231
    Purpose
    mammalian expression of Flag-tagged human Par4
    Depositor
    Shaun Coughlin
    Insert
    Par4 (F2RL3 Human)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-24 (native signal peptide)
    Promoter
    Available since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    53231-plasmid-map-sequence-id-176053

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  14. pg-nucODD-EGFP

    Plasmid
    #87212
    Purpose
    HIF-1a sequence without the bHLH domain fused to EGFP
    Depositor
    Violaine Sée
    Insert
    HIF-1a nucODD (HIF1A Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    without the bHLH domain, Q814R
    Promoter
    CMV
    Available since
    Sept. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    87212-plasmid-map-sequence-id-173860

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  16. pKG204

    Plasmid
    #63078
    Purpose
    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    HP1 alpha (CBX5 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    V151I mutation compared to GenBank reference sequ…
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63078-plasmid-map-sequence-id-134726

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  17. RCAS IkB alpha M

    Plasmid
    #12407
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  18. pKG326

    Plasmid
    #69753
    Purpose
    Retroviral expression of CENP-T aa 1-531
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-531 (CENPT Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    E.coli optimized sequence; A216T and G459R mutati…
    Promoter
    Available since
    Dec. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. CBLC

    Plasmid
    #39078
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBLC (CBLC Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    A64E
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

    Plasmid
    #107845
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in four clusters in spl…
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107845-plasmid-map-sequence-id-203697

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Showing: 5221 - 5240 of 12942 results