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TagBFP(P6f)3(P14f)3-gs

Plasmid

#228081

Purpose

Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, P6f, P14f

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

Plasmid

#109320

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCherry

Plasmid

#193060

Purpose

doxycycline-inducible expression of mCherry in mammalian cell

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc DeltaN TCF4

Plasmid

#16513

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Expression

Mammalian

Mutation

Delta N: lacks aa 1-32

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
mEmerald-PDHA1-N-10

Plasmid

#54225

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

PDHA1 (PDHA1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Plasmid

#219781

Purpose

CaST-P2A for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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NG-ABE8-dimer

Plasmid

#138497

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTU1-B-SP44s-GUS

Plasmid

#172430

Purpose

Expresses Streptomyces codon-optimised beta-D-glucuronidase

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

beta-D-glucuronidase

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

SP44s

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-EGFP_puromycin

Plasmid

#65284

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BILF2

Plasmid

#195788

Purpose

Gateway Entry Clone of Epstein-Barr virus BILF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BILF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIK

Plasmid

#111197

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

NIK (MAP3K14 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLncEXP

Plasmid

#64865

Purpose

Empty vector for lncRNA expression

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Type

Empty backbone

Use

Retroviral

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)3(S4)3-SAH

Plasmid

#228069

Purpose

Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with long rigid linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ABE8e-dimer

Plasmid

#138490

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-CRY2-OCRL

Plasmid

#79565

Purpose

expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

Depositor

Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

OCRL (OCRL Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539

Promoter

CMV

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Expression

Yeast

Promoter

TDH3

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-cTF

Plasmid

#87743

Purpose

IPTG-inducible expression of ligase-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and cTF (chimeric transcription factor base…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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