We narrowed to 16,144 results for: LEA

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pJL1

Plasmid

#184129

Purpose

For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1 (3kb)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pROSA26-G19

Plasmid

#21711

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 G19 (Gt(ROSA)26Sor Mouse)

Expression

Bacterial

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-ChRger1-TS-EYFP

Plasmid

#127244

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger1-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CamKIIa

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shPrc1

Plasmid

#160959

Purpose

Prc1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Prc1 shRNA (Prc1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CD23b in pIRES2eGFP

Plasmid

#19136

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

CD23 beta (FCER2 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Del3 C-term GFP

Plasmid

#36136

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 39-216 of cyclophilin B; G63…

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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UR1Ct (265-1040)

Plasmid

#115647

Purpose

expression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag1 (265-1040) (Rag1 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag1 (aa 265-1040)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52537

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET30b GluBP600n

Plasmid

#119835

Purpose

Production of recombinant GluBP600n high affinity protein in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

GluBP600n

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52538

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 short UTR

Plasmid

#21648

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2dark

Plasmid

#178874

Purpose

Mammalian expression of PB1-MontiRed-LOV2dark

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2dark

Expression

Mammalian

Mutation

LOV2: C450A, L514K, G528A, L531E, N538E

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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M48 GST-TEV-Chibby-N

Plasmid

#17196

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-63

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pProvector_AAV2_586_PLGLAR

Plasmid

#129024

Purpose

Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

Depositor

Junghae Suh

Article

Tong et al J Control Release. 2019 Jun 25. pii: S01

Insert

Rep2/Cap2 with PLGLAR lock at 586

Use

AAV

Mutation

inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET41a iGlul

Plasmid

#119832

Purpose

Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlul

Tags

GST

Expression

Bacterial

Mutation

T92A

Promoter

T7

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hB50

Plasmid

#18967

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

Insert

Brevican N-terminus (BCAN Human)

Expression

Mammalian

Mutation

Human brevican N-terminus aa 1-400

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOCC6

Plasmid

#118864

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease, EGFP

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA6/ERGIC2-WT

Plasmid

#51479

Purpose

Expresses ERGIC2-WT in mammalian cells

Depositor

Simon Kwok

Article

Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

Insert

ERGIC2-WT (ERGIC2 Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only