We narrowed to 8,512 results for: gnal

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RCAS-sgATG7

Plasmid

#75228

Purpose

An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

Depositor

Noor Gammoh

Article

Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Promoter

U6

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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CRAF_S29A_pcDNA3.1

Plasmid

#86506

Purpose

Expresses CRAF_S29A in mammalian cells [Edit]

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN (C terminus)

Plasmid

#72296

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN C-terminus (FLCN Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains only C terminus of FLCN as defined in PM…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pPGK-T7/2-CD44v8-10 (-cyt)

Plasmid

#137822

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1 C121S

Plasmid

#53164

Purpose

Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

C121S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 P5-6D

Plasmid

#51254

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Asp

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-hDIXDC1 S592A

Plasmid

#61223

Purpose

Mammalian expression vector encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 592 to Alanine

Promoter

CMV

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSico shMAPKAPK3 human

Plasmid

#85661

Purpose

expression of shRNA targeting human MAPKAPK3

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

MAPKAPK3 (MAPKAPK3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pECE beta1 R760A

Plasmid

#50511

Depositor

Kenneth Yamada

Article

Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

R760A

Promoter

SV40

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
RON6-bio

Plasmid

#47785

Purpose

Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RON6

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin replaced…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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LP696

Plasmid

#90177

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1289-1826

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1289-1826

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374delNLS2

Plasmid

#62532

Purpose

N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

truncated human Drosha mutant (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269 and nuclear localizatio…

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 RL3m6L2

Plasmid

#109366

Purpose

Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-bio-myc-NES-EGFP

Plasmid

#112712

Purpose

For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

EGFP

Tags

HIV Rev nuclear localization signal (NES), biotin…

Expression

Mammalian

Mutation

Nucleotide sequence optimized for synthetic gene …

Promoter

CMV with Tet repressor binding sites

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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