We narrowed to 25,487 results for: RING;

1. pCMV NucleoBAS N354K-I372V

   Plasmid

   #63574

   Purpose

   Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

   Depositor

   Stan van de Graaf

   Article

   van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

   Insert

   NucleoBAS N354K-I372V
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   N345K and I372V

   Promoter

   CMV

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. natMX-ins7

   Plasmid

   #195044

   Purpose

   pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   NatR
   

   Use

   Yeast genomic targeting

   Tags

   tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-hs-NRAS

   Plasmid

   #52728

   Purpose

   retroviral expression of human NRAS

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   NRAS (NRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCI-MS2V5-eIF4G delN83

   Plasmid

   #65812

   Purpose

   expression of eIF4G-MS2 fusion.

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eIF4G (EIF4G1 Human)
   

   Use

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   delN83 (functionally behaves as the WT protein)

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. LIC-Z-YFP

   Plasmid

   #153544

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   Venus YFP

   Expression

   Mammalian

   Mutation

   with mCherry in LIC-Z replaced by YFP

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hs-3xHA-NHL only-LIN-41

   Plasmid

   #52723

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   NHL-only contains only an initiating methionine a…

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PABPC1_544-636_pGEX6P1

   Plasmid

   #209789

   Purpose

   Bacterial expression of PABPC1 Mlle domain

   Depositor

   Kalle Gehring

   Article

   Kozlov et al EMBO J. 2004 Jan 28;23(2):272-81. doi: 1

   Insert

   Mlle domain of PABPC1 (PABPC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 544-636 only

   Promoter

   tac

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNOC_dCas9_sgRNA Td1

   Plasmid

   #176257

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNOC_hfnCas12a-Nlux_crRNA NR2

   Plasmid

   #176245

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_hfnCas12a_crRNA NR2

    Plasmid

    #176248

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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