We narrowed to 11,619 results for: ESP

1. P0050::rpaAD53A

   Plasmid

   #101831

   Purpose

   drives expression of rpaA D53A variant from an rpaA-independent promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
   

   Use

   Cloning vector

   Mutation

   D53 mutated to A

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEN_TTmiRc2 3XFLAG-EGFP-NLS

   Plasmid

   #87778

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Tags

   3xFLAG and NLS

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

   Plasmid

   #87777

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK7-EE
   

   Tags

   3xFLAG and NLS

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PHBA01

   Plasmid

   #83535

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC gene

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-UBiC-CYC1t
   

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcasper4 p [7-Gr66a-]

   Plasmid

   #26860

   Depositor

   Craig Montell

   Article

   Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

   Insert

   Gr66 gustatory receptor (Gr66a Fly)
   

   Expression

   Insect

   Mutation

   Partial deletion of Gr66a (see associated paper l…

   Available Since

   May 18, 2011

   Availability

   Academic Institutions and Nonprofits only

6. TM1442-pET-21a

   Plasmid

   #12650

   Depositor

   SeWon Suh

   Article

   Lee et al Proteins. 2004 Jul 1. 56(1):176-9.

   Insert

   Putative anti-sigma factor antagonist (TM1442 T. maritima)
   

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTorPE-Y-GECO2s

   Plasmid

   #105067

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2s
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTorPE-Y-GECO2.1s

   Plasmid

   #105068

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2.1s
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL3 - Brr2 del113 N1104L

   Plasmid

   #90253

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

   Insert

   BRR2
   

   Tags

   TAP

   Expression

   Yeast

   Mutation

   deleted first 113 amino acids and amino acid N110…

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL7 - Brr2 del247 N1104L

    Plasmid

    #90257

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 247 amino acids and N1104L

    Availability

    Academic Institutions and Nonprofits only

11. pSL11 - Brr2 del422 N1104L

    Plasmid

    #90261

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids and amino acid N110…

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSL12 - Brr2 del422 Q904E

    Plasmid

    #90262

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids and amino acid Q904E

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSL13 - Brr2 del422 R1107L

    Plasmid

    #90263

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids and amino acid R110…

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXD70LacZ1-promoterless-LacZ

    Plasmid

    #191617

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LOC5567066 Gastrin-releasing peptide receptor

    Plasmid

    #115372

    Purpose

    Expresses Ae. aegypti receptor in mammalian cells

    Depositor

    Leslie Vosshall

    Article

    Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

    Insert

    LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMH-SFB

    Plasmid

    #99391

    Purpose

    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge