We narrowed to 18,575 results for: MUT

1. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

   Plasmid

   #135456

   Purpose

   Bacterial expression of uTEV2Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, T30A/S153N mut…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJE53

   Plasmid

   #194153

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPOP-V5 (F133V)

   Plasmid

   #169725

   Purpose

   Mammalian Expression of mouse SPOP F133V

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   F133V

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-ZM197M-gp160

   Plasmid

   #123226

   Purpose

   Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (ZM197M) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-Q769gp160

   Plasmid

   #100928

   Purpose

   Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (Q769.d22) Env
   

   Tags

   CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE497

   Plasmid

   #153249

   Purpose

   M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA-trp-HYB

   Plasmid

   #64331

   Purpose

   trp1 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of trp1 deletion gRNA cassette
   

   Expression

   Yeast

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. SPOP-V5 (W131G)

   Plasmid

   #169724

   Purpose

   Mammalian Expression of mouse SPOP W131G

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   W131G

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-Flex-TC66T

    Plasmid

    #48331

    Purpose

    Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

    Insert

    TVA receptor mCherry fusion with the 66T mutation
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry fusion to TVA receptor

    Expression

    Mammalian

    Mutation

    66T

    Promoter

    CAG

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE651

    Plasmid

    #153229

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. G10 Cas9 MDC123

    Plasmid

    #59187

    Purpose

    G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    G10

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE342

    Plasmid

    #153251

    Purpose

    M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PIK3CA-WT (pFastBac)

    Plasmid

    #16644

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas9 MDC123

    Plasmid

    #59184

    Purpose

    2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    2X35S

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPOP-V5 (BACK))

    Plasmid

    #169722

    Purpose

    Mammalian Expression of mouse SPOP Y353E

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Y353E

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS416 Gal TDP43 WT

    Plasmid

    #27458

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pBSE901

    Plasmid

    #91709

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-STAG2-wildtype

    Plasmid

    #31972

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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