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pHAGE-UBC-MCP-mNeonGreen

Plasmid

#207581

Purpose

The lentiviral plasmids for expression of MCP-NeonGreen

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

MS2 coat protein mNeonGreen fusion

Use

Lentiviral

Tags

mNeonGreen

Promoter

UBC

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 N-terminal sgRNA

Plasmid

#207091

Purpose

pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGGGAGCAGATGGACCCTAC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG3

Plasmid

#193801

Purpose

Protein production of ChemoG3 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG3

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.2

Plasmid

#193803

Purpose

Protein production of ChemoG3.2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG4

Plasmid

#193804

Purpose

Protein production of ChemoG4 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG4

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: E143R, E147R, L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG1

Plasmid

#193806

Purpose

CMV overexpression of ChemoG1 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Mammalian

Mutation

EGFP: A206K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG1

Plasmid

#193799

Purpose

Protein production of ChemoG1 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG2

Plasmid

#193800

Purpose

Protein production of ChemoG2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ714

Plasmid

#132955

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-2A-GFP-IRESpuro2

Plasmid

#183746

Purpose

Expresses H2B-2A-GFP

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

GFP with 2A protease cleavage site between H2B an…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-His-sfGFP

Plasmid

#180755

Purpose

pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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Stargazin-dCherry-FRB

Plasmid

#172444

Purpose

Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cacng2 (Cacng2 Mouse)

Tags

dCherry-HA-FRB

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH462

Plasmid

#172154

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Sumo-tagged pylBCD); AraC

Expression

Bacterial

Mutation

SUMO tag fused to N-terminus of PylB

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Fyn-TagBFP

Plasmid

#171035

Purpose

TagBFP-tagged Fyn kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Fyn

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-shSCR

Plasmid

#139472

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pM116: CasRx VEGFA presgRNA

Plasmid

#166868

Purpose

U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM62624

Plasmid

#169094

Purpose

Low copy MoClo level 2 binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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