We narrowed to 7,493 results for: addgene/1000

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc P

Plasmid

#221855

Purpose

Express human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses P domain

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc N

Plasmid

#221854

Purpose

Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses N domain

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc C

Plasmid

#221856

Purpose

Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses C domain

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-VIM270-pSM

Plasmid

#215871

Purpose

This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

Depositor

Kumiko Ui-Tei

Article

Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

Insert

3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)

Expression

Mammalian

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro 2xStrep-HPC4-Gb5

Plasmid

#218177

Purpose

Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

G beta subunit 5 (GNB5 Human)

Use

Lentiviral

Tags

2x Strep tag and HPC4

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR ins5

Plasmid

#214769

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR ins5 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroPATCHY3

Plasmid

#215595

Purpose

For overexpression of Gal4-DBD EGR1 AroPATCHY3

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroPATCHY3 (EGR1 Human)

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroLITE_A

Plasmid

#215593

Purpose

For overexpression of Gal4-DBD EGR1 AroLITE A

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroLITE A (EGR1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroPATCHY1

Plasmid

#215596

Purpose

For overexpression of Gal4-DBD EGR1 AroPATCHY1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroPATCHY1 (EGR1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-GFP

Plasmid

#212815

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

8xHis and CUbo-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-His6

Plasmid

#212819

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo-6xHis

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

Plasmid

#212822

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo(K63R)

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Bn R52A, R54A pMT2

Plasmid

#206087

Purpose

Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

R52A, R54A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del18-197)-FLAG

Plasmid

#204539

Purpose

Mammalian expression of human CALR ins5 (del18-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del18-197)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del18-197)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del198-430)-FLAG

Plasmid

#204538

Purpose

Mammalian expression of human CALR ins5 (del198-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del198-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del367-430)-FLAG

Plasmid

#204536

Purpose

Mammalian expression of human CALR ins5 (del367-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del367-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del367-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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