178,377 results
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Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
ExpressionMammalianMutationD210N, catalytically inactiveAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAC565
Plasmid#195242PurposeMammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
ExpressionMammalianAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV1)
Viral Prep#162380-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SomaGCaMP6f2 (AAV8)
Viral Prep#158757-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG promoterAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Luc2-IRES-ZsGreen-W
Plasmid#164432PurposeLentiviral vector backbone encoding firefly luciferase and ZsGreenDepositorInsertsLuciferase
ZsGreen
UseLentiviralAvailable SinceFeb. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 -EGFP
Plasmid#165108Purposemammalian expression and localizationDepositorAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXG237
Plasmid#141281PurposeATF4 translational reporterDepositorInsertuORF1/2 of ATF4 followed by mApple
UseLentiviralTagsmApplePromoterCMVAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf3b-IRES-Puro
Plasmid#141384PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf3b
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro
Plasmid#141389PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf7b (ORF7b SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 TFAM-mScarlet
Plasmid#129573PurposeExpression of mScarlet fused TFAMDepositorAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
aeBlue chromoprotein
Plasmid#117846PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, aeBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGIPZ-PD-L1-EGFP
Plasmid#120933PurposeExpress PD-L1-EGFP fusion protein in mammalian cellsDepositorInsertPD-L1-EGFP
ExpressionBacterialAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BRAF-V600E
Plasmid#116204PurposeLentiviral expression of BRAF V600EDepositorAvailable SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn-FLEX.iGABASnFR.F102G (AAV1)
Viral Prep#112164-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSyn-FLEX.iGABASnFR.F102G (#112164). In addition to the viral particles, you will also receive purified pAAV.hSyn-FLEX.iGABASnFR.F102G plasmid DNA. Synapsin-driven, Cre-dependent iGABASnFR.F102G GABA sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas ServiceAAV PHP.eB and AAV5InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterGFAPAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCpf1-2NLS
Plasmid#102566Purposebacterial expression of LbCpf1-2NLSDepositorInsertLbCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-tTA
Plasmid#99120PurposeAn AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA
Plasmid#86613PurposeVector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only