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Plasmid#223797PurposeExpression of recombinant protein for purificationDepositorInsertATG13 (ATG13 Human)
UseTagsGST-TEV-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T4uvsW
Plasmid#228201PurposeExpression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealaseDepositorInsertuvsW
UseTagshexahistidine and 10 amino acid linkerExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Luke-2
Plasmid#228900PurposeLuke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertCarbohydrate binding domain cbm49 protein
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-dRBR-HA-MND-zeo
Plasmid#232802PurposeInducible over expression of RNA-binding domain deficient form of CTCF.DepositorInsertCtcf-dRBR (Ctcf Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-WT-HA-MND-zeo
Plasmid#232801PurposeInducible over expression of wild type version of CTCF.DepositorInsertCTCF (Ctcf Mouse)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-1
Plasmid#230086PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-2
Plasmid#230087PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
2xMyc-tdMCP-SNAP (tdMCP standard)
Plasmid#226166PurposeFor transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.DepositorInsert2xMyc-tdMCP-SNAPtag
UseLentiviralTags2xMyc-tdMCP and EGFP-IRES-BlastRExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TMEM16F-I611K-peGFP-N1
Plasmid#228581PurposeExpresses mouse TMEM16F-I611K-eGFP in mammalian cellsDepositorInsertTMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
UseTagsExpressionMammalianMutationI611K plus a 3 amino acid N-terminal truncation (…PromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo_BACE1
Plasmid#227105PurposeBACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assaysDepositorInsertBACE1 (BACE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mCherry
Plasmid#223952PurposeGateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
miRFP670-TRF1
Plasmid#227388PurposeLabel telomere, no adhesionDepositorInsertTelomeric miRFP (TERF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TERF1
Plasmid#222542PurposePiggyBac transposon plasmid for doxycycline inducible expression of TERF1DepositorInsertTERF1 (TERF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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TurboID-13x-EV
Plasmid#215086PurposeExpresses TurboID-13x[linker] in mammalian cells from a retroviral vector.DepositorInsertTurboID (birA )
UseRetroviralTags13x [linker]ExpressionMammalianMutationPromoterLTRAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG
Plasmid#206956PurposeAAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulinsDepositorInsertmScarlet-I, MmMT3, QtEnc
UseAAVTagsFLAGExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mPF-GFP
Plasmid#199222PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertrtTA-Advanced::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only