167,318 results

1. GFP-PKCA D463N

   Plasmid

   #195203

   Purpose

   Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

   Depositor

   David Bryant, Peter Parker

   Article

   Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

   Insert

   PRKCA (PRKCA Human)
   

   Expression

   Mammalian

   Mutation

   D463N

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHiUGE HA-epitope donor ORF0

   Plasmid

   #200371

   Purpose

   Tagging endogenously expressed proteins with HA epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   HA epitope
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFBP-2_6.sensor

   Plasmid

   #162800

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6_RNA-device
   

   Expression

   Yeast

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_DKK1_WT

   Plasmid

   #82250

   Purpose

   Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (AAV1)

   Viral Prep

   #175181-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (#175181). In addition to the viral particles, you will also receive purified pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt plasmid DNA. Synapsin-driven, Cre-dependent, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Promoter

   Syn

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

6. Dronpa3-C1

   Plasmid

   #54655

   Purpose

   Localization: C1 Cloning Vector, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   Dronpa3

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pDONR221_SLC4A7

   Plasmid

   #132204

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC4A7 (SLC4A7 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Tol2-UAS-Dendra2

   Plasmid

   #97145

   Purpose

   For Gal4/UAS expression system

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   Dendra2
   

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-Cyclin F

   Plasmid

   #215054

   Purpose

   Expresses EGFP-Cyclin F in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNF (CCNF Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104140

    Purpose

    Expresses TREAT-5'TOP reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSimpleII-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47867

    Purpose

    This plasmid encode NmCas9 with two NLS and a HA tag

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLL2787

    Plasmid

    #15460

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    phage phi11 int gene
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-Alpha-Actinin2-N-18

    Plasmid

    #54974

    Purpose

    Localization: Alpha Actinin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Alpha-Actinin2 (ACTN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Fibronectin-human-plasma in pMAX

    Plasmid

    #120401

    Purpose

    enables eukaryotic expression of human plasma fibronectin

    Depositor

    Inaam Nakchbandi

    Article

    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

    Insert

    Fibronectin (FN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge