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Hmga2 3'UTR m2 luciferase (Luc-m2)

Plasmid

#14786

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m2 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m2 mutant (see article and author's map for …

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-DsRed-cNLS

Plasmid

#138533

Purpose

Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE-CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MAP2-CD4

Plasmid

#43916

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

CD4 (CD4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xFLAG-KIF20A

Plasmid

#192297

Purpose

Gateway entry vector for an inducible 3xFLAG-KIF20A

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KIF20A (KIF20A Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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NAE1 G7.3 gRNA

Plasmid

#90776

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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PLK1 G5.1 gRNA

Plasmid

#90834

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-Gαq*-BERKY1

Plasmid

#158430

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TAL2261

Plasmid

#36700

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BMPR1A-TALEN_Right (BMPR1A Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
TAL2260

Plasmid

#36699

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BMPR1A-TALEN_Left (BMPR1A Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pTol1-UAS:Chronos-tdTomato

Plasmid

#124232

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

Chronos-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP mLST8 (aka: GbL)

Plasmid

#12575

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mLST8 (MLST8 Human)

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pX330-PITCh-TUBB4B

Plasmid

#207785

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8b in pCSDEST2

Plasmid

#140881

Purpose

Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8b (rab8b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSKA397

Plasmid

#80380

Purpose

mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

cat
metE

Use

Synthetic Biology; Mini-tn7 delivery vector

Expression

Bacterial

Promoter

cat promoter and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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FUp95GW (B7)

Plasmid

#74016

Purpose

lentiviral expression of shRNA resistant Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

Plasmid

#184633

Purpose

Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Aldh1a1 (Aldh1a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PSMD14 E6.4 gRNA

Plasmid

#90859

Purpose

3rd generation lentiviral gRNA plasmid targeting human PSMD14

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PSMD14 (Guide Designation E6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7 shRNA against CRTC2

Plasmid

#72711

Purpose

Lentiviral expression of shRNA against CRTC2 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC2

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only