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pCMV-MtPylRS (human-opti)

Plasmid

#164080

Purpose

To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Publication

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtPylRS-478(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shPrc1

Plasmid

#160959

Purpose

Prc1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Prc1 shRNA (Prc1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shMki67

Plasmid

#160958

Purpose

Mki67 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Mki67 (Mki67 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp8-HF

Plasmid

#157696

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp2-HF

Plasmid

#157684

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133672

Plasmid

#152198

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 (-) hYAP 1-2beta

Plasmid

#124144

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Publication

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]

Plasmid

#107852

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348V in splicing reporter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309F]

Plasmid

#107851

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G309F in splicing reporter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1339 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S]

Plasmid

#107850

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348S in splicing reporter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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Ptrc::RpaA D53A

Plasmid

#105229

Purpose

inducible expression of unphosphorylatable variant of RpaA (D53A)

Depositor

Erin O'Shea

Publication

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

rpaA driven by Ptrc promoter

Use

Cyanobacteria cloning vector

Mutation

D53 mutated to A

Promoter

ptrc

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Publication

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Mutation

V30M

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPQ4

Plasmid

#83559

Purpose

Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL7 promoter-yEGFP

Expression

Yeast

Promoter

GAL7

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX2-AS-long (pcDNA3.1+)

Plasmid

#75199

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Insert

SLC24A2 (Slc24a2 Mouse)

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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JW616

Plasmid

#64805

Purpose

CUC2 expression in yeast

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

GAL4 Activation Domain, HA, and SV40 NLS

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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