We narrowed to 12,076 results for: shRNA

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pLKO1-puro-U6-sgRNA-PLXNB2

Plasmid

#69240

Purpose

U6 driven SpCas9 sgRNA expression for PLXNB2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

PLXNB2 sgRNA (PLXNB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

Plasmid

#68356

Purpose

-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

Depositor

Jannik Elverløv-Jakobsen

Inserts

4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
FlpO recombinase

Use

CRISPR

Tags

mTurquoise2 (BFP)

Expression

Mammalian

Promoter

Synthetic Probasin ARRx2 and U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC103

Plasmid

#62340

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-1

Plasmid

#92159

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-2

Plasmid

#92160

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179120

Purpose

Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

control sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR

Plasmid

#111177

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro

Plasmid

#21915

Purpose

3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

Depositor

Dmitri Wiederschain

Article

Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only

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PX458_ARID5B_1

Plasmid

#86350

Purpose

Encodes gRNA for 3' target of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID5B (ARID5B Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ARID5B_2

Plasmid

#86351

Purpose

Encodes gRNA for 3' target of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID5B (ARID5B Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0760

Plasmid

#185629

Purpose

Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SGEP

Plasmid

#111170

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus

Plasmid

#25734

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a7

Plasmid

#124846

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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SGEN

Plasmid

#111171

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_526

Plasmid

#184859

Purpose

Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

Depositor

Arlene Sharpe

Article

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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