We narrowed to 2,036 results for: ARP

1. pXDC61-LepA

   Plasmid

   #21843

   Depositor

   Howard Shuman

   Article

   Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

   Insert

   LepA (lepA L. pneumophila)
   

   Use

   Tags

   TEM

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rab11A[8-175]-His-C Q70L

   Plasmid

   #228042

   Purpose

   Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

   Depositor

   Wade Harper

   Insert

   Rab11A[8-175]-His-C Q70L (RAB11A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q70L

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214996

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (TMEM115 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Use

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST17-Cdc34a

   Plasmid

   #18674

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Cdc34A (CDC34 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDA303

   Plasmid

   #195199

   Purpose

   Overexpressing 6His-TetR in E. coli.

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

   Insert

   TetR
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HC-M

   Plasmid

   #173481

   Purpose

   An rrnB P1-based GFP ATP reporter in E. coli

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

   Insert

   GFP-mut2
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   rrnB P1

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV puro let-7 sponge

    Plasmid

    #29766

    Depositor

    Phil Sharp

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    let-7 sponge
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #218633

    Purpose

    Proximity biotinylation of msNLRP3

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    UBC

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-N-Flag-HA-GFP

    Plasmid

    #41034

    Purpose

    Retroviral expression of GFP in mammalian cells

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. p4580 AU-IRF-3a

    Plasmid

    #8660

    Depositor

    Peter Howley

    Article

    Karpova et al Mol Cell Biol 2001 Jul;21(13):4169-76.

    Insert

    IRF-3a (IRF3 Human)
    

    Use

    Tags

    AU

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

15. msHook2 g1 lentiCRISPRv2-mCherry

    Plasmid

    #218652

    Purpose

    Knockout vector for mouse Hook2

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Hook2 (Hook2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 msNLRP3 (STOP)

    Plasmid

    #218638

    Purpose

    Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. msHook2 g2 lentiCRISPRv2-mCherry

    Plasmid

    #218653

    Purpose

    Knockout vector for mouse Hook2

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Hook2 (Hook2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFOSGE_COF2A07

    Plasmid

    #70902

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Arp5 (Arp5 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-DN-hCUL1-FLAG

    Plasmid

    #15818

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin1 (CUL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N452

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge