Addgene

We narrowed to 2,024 results for: ARP

Showing: 521 - 540 of 2024 results

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Use

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

rrnB P1

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214997

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (Tmem115 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214996

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (TMEM115 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV puro let-7 sponge

Plasmid

#29766

Depositor

Phil Sharp

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

let-7 sponge

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
pDEST17-Cdc34a

Plasmid

#18674

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Cdc34A (CDC34 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218633

Purpose

Proximity biotinylation of msNLRP3

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

UBC

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-N-Flag-HA-GFP

Plasmid

#41034

Purpose

Retroviral expression of GFP in mammalian cells

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral; Gateway destination vector

Tags

Flag, HA, and IRES Puro

Expression

Mammalian

Mutation

Promoter

LTR-driven expression

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
p4580 AU-IRF-3a

Plasmid

#8660

Depositor

Peter Howley

Article

Karpova et al Mol Cell Biol 2001 Jul;21(13):4169-76.

Insert

IRF-3a (IRF3 Human)

Use

Tags

AU

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA -TMEM9-3xHA

Plasmid

#225508

Purpose

TMEM9 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM9 (TMEM9 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA -TMEM9B-3xHA

Plasmid

#225509

Purpose

TMEM9B expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM9B (TMEM9B Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-TMEM30A-V5

Plasmid

#225510

Purpose

TMEM30A expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM30A (TMEM30A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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msHook2 g1 lentiCRISPRv2-mCherry

Plasmid

#218652

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msHook2 g2 lentiCRISPRv2-mCherry

Plasmid

#218653

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2A07

Plasmid

#70902

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Arp5 (Arp5 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_dCas9-5xGCN4_Hygro

Plasmid

#235572

Purpose

Dox-inducible expression of dCas9-GCN4 & genomic integration

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

dCas9-5xGCN4

Use

CRISPR

Tags

Expression

Mutation

D10A; H840A

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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