We narrowed to 609 results for: Lambda RED

1. pKDsgRNA-pheT

   Plasmid

   #154920

   Purpose

   aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting pheT
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDsgRNA-mqo

   Plasmid

   #154921

   Purpose

   aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting mqo
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-hisF

   Plasmid

   #154922

   Purpose

   aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting hisF
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDsgRNA-ampG

   Plasmid

   #154923

   Purpose

   aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting ampG
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIJ8

   Plasmid

   #68122

   Purpose

   Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Inserts

   flp recombinase
   rhaS
   rhaR
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Dec. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSLTS

   Plasmid

   #59386

   Purpose

   Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Type

   Empty backbone

   Use

   Genome engineering

   Expression

   Bacterial

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIMcpf1

   Plasmid

   #153034

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBJZ_003

   Plasmid

   #155358

   Purpose

   Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJBJZ_001

   Plasmid

   #155357

   Purpose

   Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pJBJZ_006

    Plasmid

    #155359

    Purpose

    Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pEQ111m

    Plasmid

    #40190

    Depositor

    Paul Modrich

    Article

    Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

    Insert

    EcoRI endonuclease & EcoRI methylase
    

    Expression

    Bacterial

    Mutation

    changed Glutamic Acid 111 to Glutamine

    Promoter

    Lambda PL

    Available Since

    Nov. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSU313

    Plasmid

    #169695

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKDsgRNA-rssA

    Plasmid

    #89957

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rssA gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSUB11

    Plasmid

    #169699

    Purpose

    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    

    Tags

    3xFLAG tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUB7

    Plasmid

    #169698

    Purpose

    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSU315

    Plasmid

    #169697

    Purpose

    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Tags

    1xHA tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pES134

    Plasmid

    #127551

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Article

    Wu et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    F3-flanked spectinomycin resistance cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSU314

    Plasmid

    #169696

    Purpose

    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
    

    Tags

    1xHA tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSU312

    Plasmid

    #169694

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge