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We narrowed to 1,477 results for: ccdb gene

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Showing: 521 - 540 of 1477 results

  1. pDEST-Spe4-RfA-mClover3-stop

    Plasmid
    #186389
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186389-plasmid-map-sequence-id-373834

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  2. pDEST-Spe4-Rfa-mCherry-stop

    Plasmid
    #186388
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186388-plasmid-map-sequence-id-373832

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  3. pDEST-Spe4-Kozak-mVenus-RfA

    Plasmid
    #186384
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186384-plasmid-map-sequence-id-373827

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  4. pDEST-Spe4-Kozak-mNeonGreen-RfA

    Plasmid
    #186382
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    186382-plasmid-map-sequence-id-373805

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  5. pDEST-Spe4-Kozak-mEos4b-RfA

    Plasmid
    #186380
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    186380-plasmid-map-sequence-id-373797

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  6. pDEST-Spe4-Kozak-mClover3-RfA

    Plasmid
    #186379
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186379-plasmid-map-sequence-id-373710

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  7. pDEST-Spe4-Kozak-mCherry-RfA

    Plasmid
    #186378
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186378-plasmid-map-sequence-id-373704

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  8. pJAT17

    Plasmid
    #204298
    Purpose
    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EYFP
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204298-plasmid-map-sequence-id-406176

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  9. pJAT7

    Plasmid
    #204292
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204292-plasmid-map-sequence-id-405844

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  10. pJAT71

    Plasmid
    #204305
    Purpose
    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204305-plasmid-map-sequence-id-405846

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  11. pJAT57

    Plasmid
    #204303
    Purpose
    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204303-plasmid-map-sequence-id-405488

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  12. pJAT58

    Plasmid
    #204304
    Purpose
    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204304-plasmid-map-sequence-id-406184

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  13. pDEST-Spe4-RfA-mScarlet-I-stop

    Plasmid
    #186393
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186393-plasmid-map-sequence-id-373587

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  14. pDEST-Spe4-Kozak-mScarlet-I-RfA

    Plasmid
    #186383
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186383-plasmid-map-sequence-id-373826

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  15. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Mutation
    BsaI site removed
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  17. pJAT31

    Plasmid
    #204291
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204291-plasmid-map-sequence-id-405841

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  18. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

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  19. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

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  20. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137020-plasmid-map-sequence-id-274032

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Showing: 521 - 540 of 1477 results