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Plasmid#37362DepositorInsertCK2α V66A/I174A (CSNK2A1 Human)
UseTagsHA X3ExpressionMammalianMutationV66A, I174APromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-NEMO-C54/347A
Plasmid#27268DepositorInsertNEMO (IKBKG Human)
UseTagsFLAGExpressionMammalianMutationCys54Ala Cys347AlaPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGY416
Plasmid#166660PurposeFor light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeastDepositorInsertLiCre
UseCre/LoxTagsExpressionYeastMutationCre: E340A, D341APromoterGAL1Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxTagsExpressionYeastMutationCre: E340A, D341APromoterMET17Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/A
Plasmid#48792PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygroDepositorInsertYFP-synhDAT-S/A (SLC6A3 Human)
UseTagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…PromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/D
Plasmid#48793PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygroDepositorInsertYFP-synhDAT-S/D (SLC6A3 Human)
UseTagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…PromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
CNC3G (CASANOVA-C3 with glycine linkers)
Plasmid#137192PurposeExpresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory proteinDepositorInsertCNC3G (CASANOVA-C3 with glycine linkers)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a F656A
Plasmid#53054PurposeExpresses alpha subunit of F656A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Phenylalanine 656 to AlaninePromoterSP6Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a Y652A
Plasmid#53053PurposeExpresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Tyrosine 652 to AlaninePromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK651RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SOX2_HDR
Plasmid#163752PurposegRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.DepositorInsertgRNA_SOX2 (SOX2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Gja1prom
Plasmid#188114PurposeExpresses luciferase under control of Gja1 promoter in transfected cellsDepositorInsertGja1 promoter (Gja1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterGja1 endogenous promoter up to gene start codonAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmTral_1-84_C
Plasmid#146082PurposeBacterial Expression of DmTral_1-84DepositorInsertDmTral_1-84 (tral Fly)
UseTagsExpressionBacterialMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbiG4FER4P2A(#249)
Plasmid#184070Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2-P2A
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LiOn*-CAG∞RFP
Plasmid#154019PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationAll TTAA in vector backbone were replaced by sile…PromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞RFP
Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationH210PPromoterCAGAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR LAT-FFF/Zap70 iLID-Drop
Plasmid#171032PurposeLight-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.DepositorInsertLAT (LAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST scFv [N100/13]
Plasmid#190521PurposeMammalian Expression of GST scFV. Derived from hybridoma N100/13.DepositorInsertGST (Schistosoma japonicum) recombinant scFV
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only