We narrowed to 5,323 results for: pcDNA3.1

1. pcDNA3.1_ZKSCAN1_Tau10-12K317M

   Plasmid

   #194164

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT (MAPT Human)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Promoter

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MUC2 D1

   Plasmid

   #181812

   Purpose

   Expresses human MUC2 D1, residues 21-389

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc2 D1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-R/G-ICUE

   Plasmid

   #181849

   Purpose

   ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   R/G-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-rsAKARev(T/A)

   Plasmid

   #166232

   Purpose

   Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

   Depositor

   Peter Dedecker

   Article

   Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

   Insert

   Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 506 in rsAKARev to Alanine

   Promoter

   CMV

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-V5-SPR

   Plasmid

   #127236

   Purpose

   Eukaryotic expression of SPR

   Depositor

   Kai Johnsson

   Article

   Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

   Insert

   SPR (SPR Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_SNORD2 probe

   Plasmid

   #73070

   Purpose

   antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD2 (SNORD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1_SNORD60 probe

   Plasmid

   #73071

   Purpose

   antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD60 (SNORD60 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-AMBRA1-3xFLAG

   Plasmid

   #172605

   Purpose

   Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-Cyto-CaNARi

   Plasmid

   #64849

   Purpose

   Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, Nuclear export signal (NES), and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-FLAG-SF3B1-WT

    Plasmid

    #82576

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 (wild-type)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-WT (SF3B1 Human)
    

    Use

    Tags

    FLAG peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaV1.2/pcDNA3.1~ Human

    Plasmid

    #140576

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    human CaV1.2 (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-RIα-EGFP

    Plasmid

    #181840

    Purpose

    GFP-tagged PKA RIα subunit for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1his p66shc (corrected)

    Plasmid

    #32574

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Nanoluc-ccdB

    Plasmid

    #87070

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 N-T2A-M-IRES-E

    Plasmid

    #231903

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203M in N protein

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Use

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)IRES GFP

    Plasmid

    #51406

    Purpose

    Expresses GFP from internal ribosome entry sequence, cloning component

    Depositor

    Kathleen_L Collins

    Article

    Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

    Insert

    IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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