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pX330-U6-BRD4-HaloTag-Guide-hspCas9

Plasmid

#228576

Purpose

Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

Depositor

Marcin Imielinski

Insert

BRD4 gRNA (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mef2d-exon minimal CMV pCDNA5

Plasmid

#212064

Purpose

LR vector for integration of Mef2d-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mef2d-exon (Mef2d Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK (281-473)

Plasmid

#134273

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 281-473

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N)

Plasmid

#134289

Purpose

Expresses GK5 (isoform 2)-D30N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 30 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v2

Plasmid

#134280

Purpose

Expresses GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v1

Plasmid

#134279

Purpose

Expresses GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v2

Plasmid

#134269

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v1

Plasmid

#134268

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7Jip1(282-470)

Plasmid

#51703

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted 1-281 and 471-660

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pFB-hSyn-SaCas9-mWPRE-hGHpA

Plasmid

#245385

Purpose

CRISPR-SaCas9 is used for in vivo genome-editing tool. Used to locate and cleave specific double-stranded DNA sequences.

Depositor

D. James Surmeier

Insert

saCas9

Use

AAV and CRISPR

Tags

SV40 NLS and nucleoplasmin NLS, 3xHA

Expression

Mammalian

Promoter

Synapsin

Available Since

June 18, 2026

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mta1+exon minimal CMV pCDNA5

Plasmid

#212070

Purpose

LR vector for integration of Mta1+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mta1+exon(12nt) (Mta1 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mta1-exon minimal CMV pCDNA5

Plasmid

#212071

Purpose

LR vector for integration of Mta1-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mta1+exon(12nt) (Mta1 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gatad2a+exon minimal CMV pCDNA5

Plasmid

#212050

Purpose

LR vector for integration of Gatad2a+exon(75nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gatad2a+exon(75nt) (Gatad2a Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gatad2a-exon minimal CMV pCDNA5

Plasmid

#212051

Purpose

LR vector for integration of Gatad2a-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gatad2a-exon (Gatad2a Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG L3mbtl3-exon minimal CMV pCDNA5

Plasmid

#212058

Purpose

LR vector for integration of L3mbtl3-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

L3mbtl3-exon (L3mbtl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mef2a-exon minimal CMV pCDNA5

Plasmid

#212062

Purpose

LR vector for integration of Mef2a-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mef2a-exon (Mef2a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mef2a+exon minimal CMV pCDNA5

Plasmid

#212063

Purpose

LR vector for integration of Mef2a+exon(24nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mef2a+exon(24nt) (Mef2a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Smarcc2-exon minimal CMV pCDNA5

Plasmid

#212074

Purpose

LR vector for integration of Smarcc2-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Smarcc2-exon (Smarcc2 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mef2d+exon minimal CMV pCDNA5

Plasmid

#212065

Purpose

LR vector for integration of Mef2d+exon(21nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mef2d+exon(21nt) (Mef2d Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Sap30bp-exon minimal CMV pCDNA5

Plasmid

#212072

Purpose

LR vector for integration of Sap30bp-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp-exon (Sap30bp Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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