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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mIRFP670nano3

Plasmid

#244916

Purpose

iATPSnFR2.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mIRFP670nano3

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_006_Kan/ColEI_sfGFP

Plasmid

#235972

Purpose

Kanamycin/ColEI backbone with sfGFP placeholder for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Kan/ColEI

Use

Synthetic Biology

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2-3a_004_atpA_Promoter+5'UTR_Cr

Plasmid

#235876

Purpose

atpA promoter + 5'UTR Chlamydomonas reinhardtii 2-3a

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

atpA promoter + 5'UTR

Use

Synthetic Biology

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_009_psbF_5'UTR_Cr

Plasmid

#235837

Purpose

psbF 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbF 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_005_Kan/ColEI_mScarlet-I

Plasmid

#235971

Purpose

Kanamycin/ColEI backbone with mScarlet-I placeholder for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Kan/ColEI

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_6_026_3C6SF

Plasmid

#235954

Purpose

3' Connector 6 Short Forward in the M2 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

3C6SF

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2_064_Synthetic_promoter_08

Plasmid

#235817

Purpose

Synthetic promoter 08 based on Chlamydomonas rrn16 promoter

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Synthetic promoter 08

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_030_rpoB2_3'UTR_Cr

Plasmid

#235921

Purpose

rpoB2 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rpoB2 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_005_psbH_3'Hom_Cr

Plasmid

#235939

Purpose

3' Homology psbH in the M2 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbH 3'Homology

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_013_psaB_5'UTR_Cr

Plasmid

#235840

Purpose

psaB 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaB 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3IEE_023_IEE5

Plasmid

#235869

Purpose

tscA-chlN IEE5 Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

IEE5

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_020_psaC_3'UTR_Cr

Plasmid

#235917

Purpose

psaC 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaC 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_015_psbC_5'UTR_Cr

Plasmid

#235842

Purpose

psbC 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbC 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_3_001_5'UTR_placeholder

Plasmid

#235830

Purpose

Encodes a sfGFP cassette in the 5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_032_rbcL_5'UTR_full_Nt

Plasmid

#235851

Purpose

rbcL 5'UTR full Nicotiana tabacum

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rbcL full 5'UTR Nt

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

Plasmid

#244837

Purpose

Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a-RVQ

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

Plasmid

#244844

Purpose

Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

iCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS

Plasmid

#244840

Purpose

Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

Plasmid

#243822

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551 R1598E F1658E

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS641_Flex-Cas12a-ABE

Plasmid

#244846

Purpose

Mammalian expression of Flex-Cas12a adenine base editor

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-dCas12a-ABE

Use

CRISPR

Tags

SV40 NLS-ABE8e

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q/D832A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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