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pGEX-2T-GST-RNMT

Plasmid

#112709

Purpose

Expresses N-terminally GST-tagged human RNMT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

GST and thrombin cleavage site

Expression

Bacterial

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

Plasmid

#194519

Purpose

Dual fluorescence reporter for studying protein degradation

Depositor

Tommer Ravid

Article

Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

Insert

TIF4631 leader sequence

Expression

Yeast

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING4

Plasmid

#13289

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING4 (ING4 Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP16

Plasmid

#158367

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP16 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf8

Plasmid

#158377

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf8 (ORF8 )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf7a

Plasmid

#158375

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7a (ORF7a )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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L_NES 41kDa

Plasmid

#201347

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_TGON2

Plasmid

#111677

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TGON2 (TGOLN2 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP4

Plasmid

#158355

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP4 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-Stra6L-6His

Plasmid

#174215

Purpose

Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

Depositor

Adrian Press

Article

Stafast et al ACS Appl. Polym. Mater. 2022

Insert

Stra6L (Stra6l Mouse)

Tags

6-His

Expression

Mammalian

Mutation

non

Promoter

CMV

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv2

Plasmid

#221139

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-Syn21-V5-mCherry-P10

Plasmid

#104617

Purpose

Expresses V5 tagged mCherry upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

Syn21-V5-mCherry-P10-MhcSA

Tags

V5

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP9

Plasmid

#158360

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP9 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFPC1-Ift52

Plasmid

#128875

Purpose

For mammalian expression of mouse Ift52

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Ift52 (Ift52 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP13

Plasmid

#158364

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP13 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFX-GalT-mCherry

Plasmid

#174473

Purpose

Express mCherry with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-ING5

Plasmid

#13292

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING5 (ING5 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only