We narrowed to 12,024 results for: pcdna

1. pcDNA3.1(-) HuTF-Fc-His tag

   Plasmid

   #183253

   Purpose

   Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

   Depositor

   Alexander McLellan

   Insert

   Tissue factor (F3 Human)
   

   Use

   Tags

   8xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-PKAcat(G1A)-mCherry

   Plasmid

   #181852

   Purpose

   mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PKAcat-mCherry (PRKACA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Glycine residue immediately after start site in P…

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-NLS-YFP-CCTc

   Plasmid

   #184327

   Purpose

   Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTc (CACNA1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

   Plasmid

   #181861

   Purpose

   FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (mScarlet-I)
   

   Use

   Tags

   mScarlet-I and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3-hAP1 420-440

   Plasmid

   #183643

   Purpose

   To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   truncated ATP6AP1 mutant; contains only the trans…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Prom1 Del EC2

   Plasmid

   #184025

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 273-287.

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8(-Ex19a) Del EC2 (Prom1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of exon 19a (amino acids 696-701), and d…

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Prom1 SV8 (-Ex19a)

   Plasmid

   #184012

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701).

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8(-Ex19a) (Prom1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of exon 19a (amino acids 696-701).

   Promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA T7-Gnat1 Mut-3UTR

   Plasmid

   #184027

   Purpose

   Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

   Depositor

   Peter Stoilov

   Article

   Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

   Insert

   Gnat1 (Gnat1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   TAG sites in the 3'-UTR are mutated to TGA

   Promoter

   CMV

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

   Plasmid

   #177767

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R9A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

    Plasmid

    #177768

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S95A

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

    Plasmid

    #177769

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K116A

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

    Plasmid

    #177770

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T139A

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

    Plasmid

    #177771

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K370A

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

    Plasmid

    #177772

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S374A

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

    Plasmid

    #177773

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K592A

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MS2-HA-HsSmg5-G120E_W

    Plasmid

    #147819

    Purpose

    Mammalian Expression of HsSmg5-G120E

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5-G120E (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

    Plasmid

    #147558

    Purpose

    Mammalian Expression of HsSmg7_633-1091

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg7_633-1091 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation V854I compared to the seq…

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-myc-TRBP-dsRBD2

    Plasmid

    #180602

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-myc-TRBP-dsRBD1

    Plasmid

    #180601

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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