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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGGEV_2_+nls-eGFP-HA-_+1_BK+

Plasmid

#49312

Purpose

Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-EGFP-HA

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pPTK012-3a-Inulinase-αMFΔ

Plasmid

#84971

Purpose

Type 3a, Inulinase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Inulinase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK004-2-pTPI1

Plasmid

#84963

Purpose

Type 2, pTPI1, Triose phosphate isomerase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Triose phosphate isomerase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFunnyfarm

Plasmid

#24755

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

CAT-ccdB cassette

Use

Gateway-adapted entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-fishRab8A

Plasmid

#24904

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B

Plasmid

#24907

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pYPQ230-TREX2-N

Plasmid

#244025

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

TREX2

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ166-TREX2-N

Plasmid

#244022

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas9

Use

CRISPR

Tags

TREX2

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ166-AtEXO1B-N

Plasmid

#244023

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas9

Use

CRISPR

Tags

AtEXO1B

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ230-AtEXO1B-N

Plasmid

#244024

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

AtEXO1B

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC316

Plasmid

#245772

Purpose

Allows for rap-1(G12V)::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rap-1(G12V)::mGFP

Use

Unspecified

Tags

mGFP

Mutation

G12V

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC318

Plasmid

#245774

Purpose

Allows for rac-2(G12V)::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rac-2(G12V)::mGFP

Use

Unspecified

Tags

mGFP

Mutation

G12V

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5415

Plasmid

#229030

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5444

Plasmid

#231064

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5448

Plasmid

#231065

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSakOk

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGE010-gR085

Plasmid

#188554

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR085

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWBhis03-Citrine

Plasmid

#188557

Purpose

Binary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

Citrine and MpIGPDm

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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