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pcDNA 3.2/V5-DEST NET1A V5 WT

Plasmid

#69817

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

NET1A (NET1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-rev-g2-2A-Venus

Plasmid

#71410

Purpose

This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

Depositor

William Wisden

Article

Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

Inserts

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

mouse hdc gene promoter, works as a pan neuronal …

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL80036

Plasmid

#68259

Purpose

Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

Plasmid

#229814

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDNA5-FRT/TO-eGFP-TONSL

Plasmid

#221012

Purpose

mammalian expression vector of eGFP tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-G88P3-HA-hM3Dq

Plasmid

#213972

Purpose

AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

Depositor

Zhonghua Lu

Article

Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

Insert

hM3D (Gq)

Use

AAV

Promoter

G88P3

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CCK1.0

Plasmid

#208673

Purpose

Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0

Use

AAV

Promoter

hSyn

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_NPYmut

Plasmid

#208678

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-EGFP-CAAX

Plasmid

#208684

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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1D3 HC

Plasmid

#170670

Purpose

Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

Depositor

Ronald Jemmerson, Rafel Radi

Article

Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

Insert

HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody

Expression

Mammalian

Available Since

July 12, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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1D3 LC

Plasmid

#170669

Purpose

Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

Depositor

Ronald Jemmerson, Rafel Radi

Article

Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

Insert

LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody

Expression

Mammalian

Available Since

June 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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hPER2.2-luc

Plasmid

#250937

Purpose

Lentiviral vector encoding a truncated promoter region of human PER2 gene driving a luciferase reporter

Depositor

Michelle Farkas

Article

Kalyanaraman et al Int J Mol Sci. 2025 Nov 6;26(21):10785.

Insert

PERIOD 2 Promoter (PER2 Human)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

contains 952 bp of the truncated human PER2 promo…

Promoter

PER2

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv2

Plasmid

#221139

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNES-EGFP-C1-flex-PABD-Spo20

Plasmid

#220081

Purpose

Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

Spo20 (SPO20 Budding Yeast)

Tags

X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

Expression

Mammalian

Mutation

amino acids 51-91

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIA033

Plasmid

#200420

Purpose

thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

bsPAmCherry

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LLP788_Traptavidin-EZH2-FL-CO

Plasmid

#211781

Purpose

AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-EZH2

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23A

Plasmid

#201458

Purpose

Expresses human RAD23A with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23B

Plasmid

#201459

Purpose

Expresses human RAD23B with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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