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pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209062

Purpose

Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

non-targeting sgRNA (C0111)

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Stat1Klf6

Plasmid

#188400

Purpose

Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Stat-1 (Stat1 Mouse, Escherichia coli)
Klf6 (Klf6 Mouse)

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-R/G-ICUE

Plasmid

#181849

Purpose

ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

R/G-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)

Plasmid

#180823

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-nSH2, cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Promoter

SV40

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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SspB-mCherry-DHPH-ITSN1

Plasmid

#176103

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-DNMT3L [N117/9.5R]

Plasmid

#114514

Purpose

Mammalian Expression Plasmid of anti-DNMT3L (Human). Derived from hybridoma N117/9.5.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-DNMT3L (Homo sapiens) recombinant mouse monoclonal antibody (DNMT3L Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TKN-bp1

Plasmid

#133428

Purpose

Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

SREBF1 (SREBF1 Human)

Expression

Bacterial

Mutation

SREBP1a amino acids 1-50 fused to GST

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (exon 1a/5) [N291C/22R]

Plasmid

#114563

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSG2_WT

Plasmid

#81917

Purpose

Gateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PSG2 (PSG2 Human)

Use

Gateway entry vector

Mutation

V20L; p.333_335del-NPT-ins-IQH

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMR3A_WT

Plasmid

#81887

Purpose

Gateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMR3A (SMR3A Human)

Use

Gateway entry vector

Mutation

C42R; P132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_WT

Plasmid

#81842

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPAG8_WT

Plasmid

#81848

Purpose

Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPAG8 (SPAG8 Human)

Use

Gateway entry vector

Mutation

E2D; E5G; P226L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CPXCR1_WT

Plasmid

#81819

Purpose

Gateway Donor vector containing CPXCR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CPXCR1 (CPXCR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_WT

Plasmid

#81758

Purpose

Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

R220G, C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.R253Q

Plasmid

#81724

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

R253Q; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_WT_V5

Plasmid

#83003

Purpose

Gateway Donor vector containing ZNF597, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.P226S

Plasmid

#81678

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

P226S; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.N42Y

Plasmid

#81638

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

N42Y; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.V117I

Plasmid

#81639

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

V117I; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK19_p.E88K

Plasmid

#81649

Purpose

Gateway Donor vector containing STK19, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK19 (STK19 Human)

Use

Gateway entry vector

Mutation

E88K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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