We narrowed to 171,178 results for: addgene

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pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PPP1CB C3.5 gRNA

Plasmid

#90845

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP1CB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP1CB (Guide Designation C3.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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TRIP13 H3.2 gRNA

Plasmid

#90920

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIP13

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TRIP13 (Guide Designation H3.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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p35S::IRE1a

Plasmid

#135232

Purpose

constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

IRE1a (IRE1A Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMCh-466

Plasmid

#92251

Purpose

Plasmid for expression of GST-tagged human TNRC6C CED in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690

Promoter

EF1A

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AURKA A2.1 gRNA

Plasmid

#90541

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKA (Guide Designation A2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_H2B

Plasmid

#65702

Purpose

Expresses YNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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GMNN D1.2 gRNA

Plasmid

#90696

Purpose

3rd generation lentiviral gRNA plasmid targeting human GMNN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

GMNN (Guide Designation D1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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UBA1 G5.3 gRNA

Plasmid

#90932

Purpose

3rd generation lentiviral gRNA plasmid targeting human UBA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UBA1 (Guide Designation G5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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WRB_pCSdest

Plasmid

#53856

Purpose

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

WRB (GET1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
RNASEH2A B9.3 gRNA

Plasmid

#90878

Purpose

3rd generation lentiviral gRNA plasmid targeting human RNASEH2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RNASEH2A (Guide Designation B9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Mito

Plasmid

#65670

Purpose

Expresses Mito-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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CLTC G10.4 gRNA

Plasmid

#90635

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLTC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CLTC (Guide Designation G10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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SRSF2 A9.3 gRNA

Plasmid

#90902

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SRSF2 A10.3 gRNA

Plasmid

#90903

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R5C B11.5 gRNA

Plasmid

#90853

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5C (Guide Designation B11.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pIS1 Cyclin D2 long-mut-miR15/16

Plasmid

#21647

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 long-mut-miR15/16 (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR15/16 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-C/pIRES puro Glue (S778-A1056)

Plasmid

#73456

Purpose

Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-N/pIRES puro Glue (M1-A497)

Plasmid

#73454

Purpose

Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, Ha tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only