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pLV-H1-SGIPZ_NF1 sh1miR

Plasmid

#119206

Purpose

knockdown of NF1

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

sh1miR targeting NF1 (NF1 Rat, Human, Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-NS-MCS

Plasmid

#75179

Purpose

mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mCherry-NS-MCS

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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EmptyTranscriptionalReporter

Plasmid

#62453

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt11-V5

Plasmid

#35941

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MKK7

Plasmid

#29576

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK7 (Map2k7 Mouse)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Starting Met removed

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
p3E-rab5c-DN

Plasmid

#109737

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(22/19), AB(monomer), wt

Plasmid

#22380

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His and myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pAR3040-spitz

Plasmid

#38341

Depositor

Norbert Perrimon

Article

Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

Insert

spitz (spi Fly)

Expression

Bacterial

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt9B

Plasmid

#35919

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Expression

Mammalian

Mutation

SNP Met106-->Thr

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5b2_Ptet

Plasmid

#179342

Purpose

gRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pSicoR mouse Dicer3

Plasmid

#14781

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
NmeCas9_3XNLS_pMCSG7

Plasmid

#120078

Purpose

Bacterial expression of wtNmeCas9 with 3X NLS

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

NmeCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-FOXI3

Plasmid

#153128

Purpose

Expression of Flag tagged human FOXI3in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXI3 (FOXI3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hmga2 m7

Plasmid

#14792

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 m7 (Hmga2 Mouse)

Expression

Mammalian

Mutation

Hmga2 m7 mutant (see article and author's ma…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
EGFP-Rab8b in pCSDEST2

Plasmid

#140881

Purpose

Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8b (rab8b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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P74-26 Tail Tube Protein (gp93) in pSMT3

Plasmid

#191261

Purpose

Expresses P74-26 gp93 with a His-SUMO tag in bacterial cells

Depositor

Brian Kelch

Article

Agnello et al J Biol Chem. 2023 Feb 13:103021. doi: 10

Insert

P74-26 gp93

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-i1 ONE-GO

Plasmid

#204328

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-i1 ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T-3-SH3BP1-CTD

Plasmid

#136649

Purpose

Expression of SH3BP1-CTD-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-CTD (SH3BP1 Human)

Expression

Bacterial

Mutation

Contains only C-terminal domain

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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