We narrowed to 53,696 results for: fer

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pAC8_MF-p10-mCh (VE5626)

Plasmid

#139772

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

mCherry fluorescent protein

Promoter

PH

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6

Plasmid

#170857

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRL-TK 3'FBXW7 UTR

Plasmid

#26649

Depositor

Alex Minella

Article

Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

Insert

FBXW7 (FBXW7 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains only the 3'UTR of FBXW7, nucleotide…

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only
1037 pGL3 FasL promoter

Plasmid

#9028

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
Stargazin-dCherry-FRB

Plasmid

#172444

Purpose

Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cacng2 (Cacng2 Mouse)

Tags

dCherry-HA-FRB

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAQ482A

Plasmid

#128945

Purpose

expresses Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2

Plasmid

#170853

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT2

Plasmid

#53558

Purpose

bacterial expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-INPP4B C842A mutant

Plasmid

#161996

Purpose

Mammalian expression vector containing a phosphatase dead mutant of INPP4B (C842A). Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP4B (INPP4B Human)

Tags

mCherry

Expression

Mammalian

Mutation

C842A

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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nLuc_AP10_PPVs_P9mS

Plasmid

#119302

Purpose

Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-DysF

Plasmid

#109492

Purpose

Muscle specific zebrafish expression of mKate2 fused to dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

DysF (dysf Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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VanGlow-GL-DSCP

Plasmid

#83338

Purpose

for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

Depositor

Cheng-Yu Lee

Article

Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

Type

Empty backbone

Use

Luciferase

Tags

GFP::Luciferase(nls)

Expression

Insect

Promoter

Drosophila Synthetic Core Promoter

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer1

Plasmid

#138573

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 enhancer (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-6

Plasmid

#14969

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/+334 (relative to the P2 transcription initi…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#163698

Purpose

AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

Depositor

Gina Turrigiano

Article

Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

Insert

CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mammalian

Promoter

CaMKII fragment

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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CDK2-3XFlag-pBabe

Plasmid

#73557

Purpose

vector encoding a fusion protein between CDK2 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK2 (CDK2 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only
(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Plasmid

#160742

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETC-RdgBbeta-splice variant 1

Plasmid

#58276

Purpose

bacterial expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only