We narrowed to 8,227 results for: sars

1. p426-Cup1p-FUS-FusionRed

   Plasmid

   #188393

   Purpose

   Cu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cells

   Depositor

   Michihiko Kataoka

   Article

   Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   FusionRed

   Expression

   Yeast

   Mutation

   Promoter

   CUP1 promoter

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMEXneo-TrkC-TrkB-hybrid

   Plasmid

   #190205

   Purpose

   To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The second IgG like domain and the extracellular …

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLY54

   Plasmid

   #130923

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GST-FUS ΔNLS 1-513

   Plasmid

   #44984

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS ΔNLS (1-513aa) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acids 1-513

   Promoter

   tac

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pEAHISMRSA2028

   Plasmid

   #97001

   Purpose

   Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP-FUSN-mHP1alpha

   Plasmid

   #181897

   Purpose

   Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   FUSN-mHP1alpha (Cbx5 Human, Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast HA-GPAT4-ΔNs5

   Plasmid

   #173169

   Purpose

   Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Synonymous mutations at sgRNA sites, amino acids …

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY9

   Plasmid

   #130907

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-2G6, and Ptet

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY59

   Plasmid

   #130927

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   sfgfp
   pspFΔHTH::MCP
   

   Use

   Synthetic Biology

   Tags

   ASV tag and MCP (MS2 coat protein)

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-2G6, and Ptet

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAav-IQGAP1-PQS1-3xFLAG

    Plasmid

    #84885

    Purpose

    rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Inserts

    IQGAP1 genomic region HR1 (IQGAP1 Human)
    IQGAP1 genomic region HR2
    

    Use

    AAV

    Tags

    PQS1 3xFLAG

    Expression

    Mutation

    Promoter

    no

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Toronto KnockOut version 3 library (TKOv3)

    Pooled Library

    #90294

    Purpose

    Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

    Depositor

    Jason Moffat

    Article

    Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223-SRC

    Plasmid

    #23934

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SRC (SRC Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223-FES

    Plasmid

    #23876

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FES (FES Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. PGL4.11-COL1A1mSMAD

    Plasmid

    #230917

    Purpose

    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant potential SMAD bindin…

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223-LYN

    Plasmid

    #23905

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    LYN (LYN Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDONR223-CKMT2

    Plasmid

    #23746

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CKMT2 (CKMT2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S6P

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pDONR223-ARAF

    Plasmid

    #23725

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ARAF (ARAF Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pDONR223-CRKL

    Plasmid

    #23354

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CRKL (CRKL Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pWZL Neo Myr Flag FGR

    Plasmid

    #20487

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    FGR (FGR Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPHR-mCherry-FUSN-mHP1alpha

    Plasmid

    #181892

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLY70

    Plasmid

    #130948

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Pcon, PpspA-LEA3B3, PrhaB, and Ptet

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWZL Neo Myr Flag CKMT2

    Plasmid

    #20554

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CKMT2 (CKMT2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pLY61

    Plasmid

    #130928

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_CASP8_WT_V5

    Plasmid

    #82999

    Purpose

    Gateway Donor vector containing CASP8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CASP8 (CASP8 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    AA 184_191delERSSSLEGinsGEELCGVMTISD; D194R; 196_…

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PGL4.11-COL1A1mTEAD

    Plasmid

    #230916

    Purpose

    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase- luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    mutant potential TEAD bind elements AGGAAT to CTG…

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223-FGR

    Plasmid

    #23877

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FGR (FGR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only