We narrowed to 25,965 results for: crispr
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Plasmid#113423PurposeExpresses hA3A-eBE-Y130F in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJZ012_EFS-Cas12a2-puro
Plasmid#257716PurposeExpress SuCas12a2 in mammalian cell lines; Puromycin selection.DepositorInsertSuCas12a2
UseCRISPRTagsNLS-FLAGExpressionMammalianPromoterEF-1alphaAvailable sinceJuly 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005
Pooled library#161769PurposeTet-inducible inhibition library targeting the yeast genome with barcode linked guide RNAs.DepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10875
Plasmid#183963PurposeCBA-hyperdCas12a-HA-KRAB-mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHyperdCas12a
UseCRISPRTagsHA, KRAB, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCBAAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAD21 (RAD21 Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dPspCas13b-wtFTO
Plasmid#223696PurposeExpressing fusion protein of dPspCas13b and wild type human FTODepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdPspCas13b-GGS-wtFTO (FTO Human)
UseCRISPRExpressionMammalianAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRExpressionBacterialPromoterPLspAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS316-RGR-GFP-mHDV
Plasmid#51058PurposeSame as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHH
Plasmid#51057PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdSaCas9
TagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDT-sgRNA
Plasmid#138271PurposeDual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)DepositorInsertsgBG, sgTS
UseCRISPRExpressionMammalianAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK392 (mAID-mClover-BSD)
Plasmid#121193PurposeC-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mClover-BS
UseCRISPRAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)
Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFlpStop-HDR-UAS-2.1-tdTom
Plasmid#89148PurposeFlpStop plasmid for CRISPR/HDR. Has MCS for homology armsDepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoter5XUAS, 3XP3-dsRedAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Plasmid#121184PurposeOsTIR1 introductionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV-OsTIR1-loxP-PURO-loxP
UseCRISPRMutationOsTIR1 codon optimized for human cellsAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJJR83
Plasmid#75028PurposemCherry^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmCherry^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized mCherryExpressionWormAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD268
Plasmid#132523PurposemNG^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mNGExpressionWormAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FNLCR CRISPRa Cell Surface
Pooled library#207471PurposeCRISPR-based activation library with guide RNAs targeting all cell surface proteins.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only