We narrowed to 10,639 results for: ESP

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PLK1 G6.1 gRNA

Plasmid

#90835

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero7

Plasmid

#187926

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero7

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero7 (SERF2 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pD765 FDFT1 3'UTR

Plasmid

#105765

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

FDFT1 3'UTR (FDFT1 Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eYFP-3x-miR204-5p-TS

Plasmid

#117380

Purpose

An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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BCL6-H2Kk-RV (H2K-BCL6)

Plasmid

#40352

Depositor

Alexander Dent

Article

Mondal et al J Immunol. 2010 Apr 15;184(8):4123-32. E

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES mouse H2Kk

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
MMTV-VEGF

Plasmid

#44625

Depositor

Robert Oshima

Article

Oshima et al Cancer Res. 2004 Jan 1;64(1):169-79.

Insert

VEGF121 (Vegfa Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

contains aa160-368; G165E mutation

Promoter

Mouse mammary tumor virus promoter

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-UIMmut

Plasmid

#69923

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Expression

Mammalian

Mutation

S224P

Promoter

CMV

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
CEP55 B12.2 gRNA

Plasmid

#90623

Purpose

3rd generation lentiviral gRNA plasmid targeting human CEP55

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CEP55 (Guide Designation B12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Nov. 15, 2017

Availability

Academic Institutions and Nonprofits only

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CEP55 B11.2 gRNA

Plasmid

#90622

Purpose

3rd generation lentiviral gRNA plasmid targeting human CEP55

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CEP55 (Guide Designation B11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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ATAD2 D8.3 gRNA

Plasmid

#90539

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATAD2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ATAD2 (Guide Designation D8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F10.4 gRNA

Plasmid

#90941

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F9.4 gRNA

Plasmid

#90940

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPP300-T2A-GFP-shP53

Plasmid

#102896

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPP300-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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POT1 E3.4 gRNA

Plasmid

#90842

Purpose

3rd generation lentiviral gRNA plasmid targeting human POT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

POT1 (Guide Designation E3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 A8.4 gRNA

Plasmid

#90897

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 A7.4 gRNA

Plasmid

#90896

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H8.1 gRNA

Plasmid

#90895

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H8.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H7.1 gRNA

Plasmid

#90894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H7.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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