We narrowed to 7,663 results for: ski

1. pSNAP-CAPZ

   Plasmid

   #69948

   Purpose

   Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

   Depositor

   Jeff Gelles

   Article

   Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

   Inserts

   CapZ beta 1 (CAPZB Chicken)
   CapZ alpha 1 (CAPZA1 Chicken)
   

   Tags

   His 6 and SNAP

   Expression

   Bacterial

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AT4G23740 J11_pECIA2

   Plasmid

   #114998

   Purpose

   Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT4G23740 (AT4G23740 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

   Plasmid

   #157716

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGEX4T3-HA-ERK2 K54R

   Plasmid

   #53170

   Purpose

   Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   K54R

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pWPI-LUC7L-3xFLAG

   Plasmid

   #201640

   Purpose

   Over-expression of 3xFLAG-tagged human LUC7L

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L (LUC7L Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCVhyg-HA-FLAG_Ubc9_WT

   Plasmid

   #164936

   Purpose

   Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   human UBC9 (UBE2I Human)
   

   Use

   Retroviral

   Tags

   FLAG & HA tags

   Promoter

   PGK promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIBN-hTRF2-tagRFP-T

   Plasmid

   #103812

   Purpose

   BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   hTRF2: deletion of amino acids 1-42 and 476 compa…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AcGFP-MLH1

   Plasmid

   #215120

   Purpose

   Expresses AcGFP-MLH1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   MLH1 (MLH1 Human)
   

   Use

   Retroviral

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106202

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PE xCas9

    Plasmid

    #172652

    Purpose

    All-in-one prime editor piggyBac transposon (xCas9)

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A-HA-Jag1

    Plasmid

    #46052

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Entry vector

    Tags

    HA tag

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pL40C_PGKintron_Cas9_Green

    Plasmid

    #134966

    Purpose

    Lentiviral vector coding Cas9 and mNeonGreen

    Depositor

    Beat Bornhauser

    Article

    Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

    Insert

    Cas9-P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    hPGK promoter with beta-globin intron

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroX-mCRT-GPI-IRES-DsRed

    Plasmid

    #223711

    Purpose

    Mammalian expression of mouse CRT-GPI

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin-GPI (Calr Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJET1.2_Gli3_Anti-Sense

    Plasmid

    #124440

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    GLI-Kruppel family member GLI3 (Gli3 Mouse)
    

    Use

    In situ probe

    Promoter

    T7

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX Abl-SH2

    Plasmid

    #46402

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Abl (ABL1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 120-225

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Elf5 gRNA

    Plasmid

    #128836

    Purpose

    gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    mElf5 gRNA (Elf5 Mouse)
    

    Use

    CRISPR

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX311 HRAS

    Plasmid

    #117749

    Purpose

    Open reading frame vector encoding HRAS

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    RASH1 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Closed vector so will not read through the V5 reg…

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZLblasti-HA-ERK2 R67S

    Plasmid

    #53172

    Purpose

    Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Mustard Weed, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R67S

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-CRT-Fc FL

    Plasmid

    #221853

    Purpose

    Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    calreticulin (CALR Human)
    

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SIN40C.SFFV.ARID3a.IRES.GFP

    Plasmid

    #169300

    Purpose

    Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    ARID3A (ARID3A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Promoter

    SFFV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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