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pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Expression

Yeast

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPTK012-3a-Inulinase-αMFΔ

Plasmid

#84971

Purpose

Type 3a, Inulinase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Inulinase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFunnyfarm

Plasmid

#24755

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

CAT-ccdB cassette

Use

Gateway-adapted entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCD294.9

Plasmid

#113319

Purpose

dCas9, PCP-SoxS_R93A, J109 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_2

Plasmid

#63803

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBbB4c-GFP

Plasmid

#35350

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS4c-RFP

Plasmid

#35302

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_52

Plasmid

#63808

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_37

Plasmid

#63807

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1

Plasmid

#91942

Purpose

Bacterial expression of maize SAE1

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

HA-ZmSAE1

Tags

HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGC276

Plasmid

#19637

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBXNAC3H

Plasmid

#47074

Purpose

FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGC163

Plasmid

#19635

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC225

Plasmid

#19636

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pBbA3c-RFP

Plasmid

#35308

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pNH043

Plasmid

#42155

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBbE4c-RFP

Plasmid

#35296

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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