We narrowed to 28,302 results for: She

1. hGli2 flag3x

   Plasmid

   #84920

   Purpose

   mammalian expression of Gli2

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli2 (GLI2 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-hM4D(Gi)-mCherry

   Plasmid

   #50475

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330.puro

   Plasmid

   #110403

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Use

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21a-alpha-synuclein

   Plasmid

   #51486

   Purpose

   Inducible expression of human alpha-synuclein in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pD454-SR mouse Alpha-synuclein

   Plasmid

   #89075

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

   Plasmid

   #39299

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV6-Bmal-luc

   Plasmid

   #68833

   Purpose

   Expression of Bmal1-luc in mammalian cells

   Depositor

   Steven Brown

   Insert

   Bmal1 regulatory sequences (Bmal1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FKBP-V5-AP-nes_pLX304

   Plasmid

   #120912

   Purpose

   Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   FKBP-V5-AP-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. human EB1 fused to monomeric RFP

    Plasmid

    #39323

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Inserts

    EB1 (MAPRE1 Human)
    mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV asyn S129A

    Plasmid

    #36066

    Depositor

    Patrick Aebischer, Hilal Lashuel

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129A

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSNAPf-H2B Control Plasmid

    Plasmid

    #101124

    Purpose

    Expression of SNAP-tagged Histone H2B in mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. TH

    Plasmid

    #39079

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TH (TH Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid

    #73333

    Purpose

    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMS2-GFP

    Plasmid

    #27121

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Use

    Tags

    EGFP, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-MAP3K5 (WT)

    Plasmid

    #47104

    Purpose

    Expresses wild type human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51087

    Purpose

    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa 1.3 kb

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103 IQGAP1 WW domain

    Plasmid

    #104279

    Purpose

    Bacterial expression of WW domain from IQGAP1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    IQGAP1 WW domain (IQGAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge