We narrowed to 8,583 results for: construct
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Plasmid#111644PurposeFor construction of spacer and repeat arrays for Sulfolobus islandicus REY154ADepositorTypeEmpty backboneUseArchaeal expression vectorPromoteraraSAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAGH42
Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialPromoternative promoter of sll0617Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30del
Plasmid#106902PurposepQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-K40R
Plasmid#64059PurposeEGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEP110E
Plasmid#97396Purposebinary construct of nuclear mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
TagsNucleus targetExpressionPlantAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f-AAV
Plasmid#182501PurposeGFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotorDepositorInsertER localized P450
UseAAVAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pES.42.1c
Plasmid#69576PurposeTCR a chain cDNA in pES4 (transgenic construct) for creating OT-1 miceDepositorAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEP104E
Plasmid#97390Purposebinary construct of mitochondria-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
Tagsmitochondria targetExpressionPlantAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC201B
Plasmid#52193Purposefor creating reversible targeting constructDepositorInsertpromoter-trap cassette Neo-Ires-GFP
UseCre/LoxTagsNeo, GFPAvailable SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBMN-mEGFP-C1
Plasmid#110468Purposeretrovirus construct for stable expressing mEGFP tagged proteinDepositorInsertmEGFP
UseRetroviral; RetrovirusTagsmEGFPExpressionMammalianAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PEP112E
Plasmid#97398Purposebinary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
TagsMitochondria targetExpressionPlantAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
TagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…Available SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv2
Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialPromoterlacIq-PtacAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-stuffer-1397N
Plasmid#187172Purposeoriginal vector to make all constructsDepositorInsertRight-stuffer-1397N
ExpressionMammalianPromoterpCMVAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
TagsHaloTagExpressionBacterialPromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv1
Plasmid#221138PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.DepositorInsert3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
UseCRISPRTags3xFLAGExpressionBacterialPromoterlacIq-PtacAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSynapsin flexed SV-tag
Plasmid#163686Purposeexpress SV-Tag construct in a Cre-dependent mannerDepositorInsertsynaptophysin 9X HA
UseAAVTags9X HAExpressionMammalianPromotersynapsinAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAE03
Plasmid#61044PurposeTo construct C-terminal GFP fusions, single crossover integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialAvailable SinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only