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pCDNA3.0_nucLAMA-G97 IRES EGFP

Plasmid

#136620

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30 PTBP1 isoform1

Plasmid

#108591

Purpose

Recombinant His tagged protein production

Depositor

Chris Smith

Article

Wollerton et al RNA. 2001 Jun;7(6):819-32.

Insert

PTBP1 isoform 1 (PTBP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

isoform 1 (isoform c)

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin A

Plasmid

#8959

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin A (CCNA2 Human)

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pPOTv7-puro-OsAID (p5109)

Plasmid

#239359

Purpose

pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Rice Auxin Inducible Degradation domain

Use

Bacterial

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQTEV-VPS28

Plasmid

#34803

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

vacuolar protein sorting 28 (yeast) (VPS28 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLP2

Plasmid

#209989

Purpose

Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PV-NES-GFP

Plasmid

#17301

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-NES-GFP (Pvalb Rat)

Tags

Myc

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-F98 IRES EGFP

Plasmid

#136618

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-tdTomato

Plasmid

#73462

Purpose

Expresses tdTomato for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

tdTomato

Use

Neurotropic virus

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416L

Plasmid

#81036

Purpose

mammalian expression of LOV V416L, a mutant with super long relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416L

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416L

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Mut2-RS

Plasmid

#182882

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut2-RS

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_35bpLinker

Plasmid

#157790

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_35bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Article

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Pepper-RhoBAST

Plasmid

#196343

Purpose

Overexpression of circular Pepper-RhoBAST

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

Tornado-Pepper-RhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Ets2

Plasmid

#28128

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98

Plasmid

#130704

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-H2GH

Plasmid

#182202

Purpose

Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

histone2B-HaloTag-GFP

Use

AAV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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