We narrowed to 24,964 results for: pET

1. pET51b(+)_LAMA-N95 (for GFP)

   Plasmid

   #130716

   Purpose

   Plasmid encoding the bacterial expression of LAMA-N95 for GFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   LAMA-N95
   

   Use

   Tags

   Hisx10

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a(+) LdtMt2 (131-408)

   Plasmid

   #59337

   Purpose

   Expresses LdtMt2 for L,D-transpeptidation

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   LdtMt2 (131-408)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET51b-Strep-SsOGT-H5-His

   Plasmid

   #167277

   Purpose

   Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SsOGT-H5
   

   Use

   Tags

   His x10 and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-11a (GB1 WT - A34F)

   Plasmid

   #136609

   Purpose

   Expresses GB1 WT mutant (A34F)

   Depositor

   Roberto Chica

   Article

   Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

   Insert

   GB1 WT mutant (A34F)
   

   Use

   Tags

   6x-Histag

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: A34F

   Promoter

   T7 promoter

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PKAsub-YPet-NES

   Plasmid

   #138203

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-JNKsubDD-YPet-NES

   Plasmid

   #84633

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsubDD-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-Vv4CL-PhCHS-CmCHI

   Plasmid

   #73401

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Vv4CL
   PhCHS
   CmCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-TALE-mCherry-15b

   Plasmid

   #138287

   Purpose

   Express tTALE-mCherry

   Depositor

   Kyubong Jo

   Article

   Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

   Insert

   7-mer tTALE-mCherry
   

   Use

   TALEN

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM6-Pc4CL-PhCHS-MsCHI

   Plasmid

   #73396

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Pc4CL
   PhCHS
   MsCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Galectin-8 linker deletion

    Plasmid

    #68806

    Purpose

    Express Galectin-8 linker deletion protein in Bacterial cells

    Depositor

    Hyun Kyu Song

    Article

    Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

    Insert

    Galectin-8 (LGALS8 Human)
    

    Use

    Tags

    N-terminal 6-histidine-tag

    Expression

    Bacterial

    Mutation

    No mutation

    Promoter

    T7

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-DEUT RUVBL1 Delete

    Plasmid

    #159144

    Purpose

    Expression and protien purification of mutated RUVBL1

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acid 1-463, Glu134-Glu237 mutated to GPPG

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b/EYFP-GgVcl 1-851

    Plasmid

    #46264

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Use

    Tags

    EYFP and His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a(+)-MVK mazei

    Plasmid

    #108947

    Purpose

    heterologous expression of MVK of M. mazei in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MBP-(CBX1)3-eReader

    Plasmid

    #135090

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered x3
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-His6-Ubc9-C138A

    Plasmid

    #133911

    Purpose

    Bacterial expression of His6-Ubc9-C138A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 138 to Alanine

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-22b(+)-RNase HIII

    Plasmid

    #12683

    Depositor

    SeWon Suh

    Article

    Kwak et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    RNase HIII
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-DEST42-SARA-V5-His6

    Plasmid

    #67431

    Purpose

    Expression of hSara with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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