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pYLL201

Plasmid

#53454

Purpose

Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Publication

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
223 pCS EGFP DEST

Plasmid

#13071

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Expression

Bacterial

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNIC-CTHF

Plasmid

#26105

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

TEV - His6 - Flag

Expression

Bacterial

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL4-ERSE2-luc2P-Hygro

Plasmid

#101790

Purpose

Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

Depositor

Seiichi Oyadomari

Publication

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xERSE-II

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hsyn-flex-dsRed-shvgat

Plasmid

#67845

Purpose

Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

Depositor

William Wisden

Publication

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

h-synapsin

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

Plasmid

#51110

Purpose

Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore

Depositor

Jonathan Ting

Insert

eArchT3.0

Use

AAV

Tags

P2A-EGFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Publication

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
Tubp-Gal4 in pCaSpeR4

Plasmid

#17747

Depositor

Liqun Luo

Publication

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL4 (GAL4 Budding Yeast)

Tags

Tubulin 1alpha promoter and hsp70 PolyA

Expression

Insect

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pYESir2HY-puro

Plasmid

#1770

Depositor

Bob Weinberg

Publication

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

H363Y, residue 363 changed from histidine to tyro…

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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LZRS ERTm RasV12

Plasmid

#21199

Depositor

Paul Khavari

Publication

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

EsR1- HRas (Esr1, HRAS Mouse, Human)

Use

Retroviral

Mutation

Esr1 G257R; HRasG12V ERTm bx3

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCR-Flag-IKKalpha-KM

Plasmid

#15468

Depositor

Hiroyasu Nakano

Publication

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha-KM (Chuk Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pRDA_479

Plasmid

#179099

Purpose

BE Cas expression

Depositor

John Doench, David Root

Publication

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (SpG)

Use

CRISPR and Lentiviral

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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repressilator (pZS1-lTlrLLtCL)

Plasmid

#26489

Depositor

Michael Elowitz

Publication

Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Insert

LacI, tetR

Expression

Bacterial

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-centrin-1

Plasmid

#72641

Purpose

The plasmid encodes centrin-1 with an N-terminal EGFP tag

Depositor

Michel Bornens

Insert

centrin-1 (CETN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcFLAG-FYN-WT

Plasmid

#74509

Purpose

Overexpression of FYN in mammalian cells

Depositor

Lars Rönnstrand

Publication

Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

Insert

FYN (FYN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK5-FLAG-Sestrin2

Plasmid

#72595

Purpose

overexpression

Depositor

David Sabatini

Publication

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles