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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 5541 - 5560 of 10083 results

  1. 6xHis-MBP-huHkCas12a

    Plasmid
    #174683
    Purpose
    Expresses 6xHis-MBP tagged HkCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huHkCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174683-plasmid-map-sequence-id-346190

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  10. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  11. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  12. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  13. K406

    Plasmid
    #129764
    Purpose
    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 406
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129764-plasmid-map-sequence-id-263574

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  14. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  15. pZF-BCB-PDZ

    Plasmid
    #127478
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-PDZ
    Depositor
    Sebastian Maerkl
    Publication
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    Use
    Cell-free transcription–translation
    Tags
    alpha 1-syntrophin PDZ domain
    Promoter
    T7
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127478-plasmid-map-sequence-id-248851

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  20. pR1G108CP20X

    Plasmid
    #85086
    Purpose
    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Publication
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1
    Promoter
    T7 promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    85086-plasmid-map-sequence-id-164463

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Showing: 5541 - 5560 of 10083 results