Addgene

We narrowed to 15,325 results for: LEA

Showing: 5561 - 5580 of 15325 results

Results per page:

Sort results by:

pET26b-SET

Plasmid

#8822

Depositor

Judy Lieberman

Article

Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

Insert

SET (SET Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pSCL.175

Plasmid

#184996

Purpose

Express -Eco1 HEK3 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

HEK3 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mPCSK8

Plasmid

#122677

Purpose

Expresses mouse PCSK8 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SynI-CreOn-FlpOff-EGFP

Plasmid

#176274

Purpose

Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin I

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-LGC-53

Plasmid

#86399

Purpose

Mammalian expression of codon-optimized dopamine receptor LGC-53

Depositor

Steven Mennerick

Article

Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

Insert

LGC-53 (lgc-53 Synthetic, Nematode)

Use

Tags

Expression

Mammalian

Mutation

Codon optimized for mammalian expression; Signal …

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178575

Purpose

Lentiviral vector for GFAP::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

GFAP

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV Tmbim6-V5

Plasmid

#175150

Purpose

Lentiviral expression of mouse Tmbim6-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmbim6 (Tmbim6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEB-Cas9-VirD2 (PCO)

Plasmid

#139802

Purpose

Expressing Cas9-VirD fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

Cas9-VirD2

Use

Tags

Cas9-VirD2

Expression

Plant

Mutation

Promoter

Ubiquitin

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.177

Plasmid

#184998

Purpose

Express -Eco1 EMX1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

EMX1 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCherry F8-2

Plasmid

#186034

Purpose

Express VHH F8-2 as a mCherry fusion in mammalian cells

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAintron-CHIKV-nsp1-c3xFLAG

Plasmid

#215694

Purpose

Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp1

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNAintron-CHIKV-nsp2-c3xFLAG

Plasmid

#215695

Purpose

Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp2

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNAintron-CHIKV-nsp4-c3xFLAG

Plasmid

#215697

Purpose

Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp4

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSCL.180

Plasmid

#185001

Purpose

Express -Eco1 AAVS1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

AAVS1 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NHA-hnRNPK

Plasmid

#120389

Purpose

Expression of HA tagged hnRNPK-lambdaN fusion

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

hnRNPK (HNRNPK Human)

Use

Tags

HA and LambdaN

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge