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pCas-Cas12m-ΔZF

Plasmid

#192276

Purpose

Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

Cas12m ΔZF

Use

CRISPR

Expression

Bacterial

Mutation

H459A, C552A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m-ΔZF

Plasmid

#192277

Purpose

pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m ΔZF

Use

CRISPR

Expression

Bacterial

Mutation

D485A, H549A, C552A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot9_19-285-Y134DG141W_AE

Plasmid

#148618

Purpose

Bacterial Expression of HsNot9_19-285-Y134DG141W

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot9_19-285-Y134DG141W (CNOT9 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC

Plasmid

#148475

Purpose

Insect Expression of DmNot1_1149-1376

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1149-1376 (Not1 Fly)

Expression

Insect

Mutation

1 silent mutation compared to the sequence given …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HsNot1iso1_1318-1605-HA-MBP_AD

Plasmid

#148503

Purpose

Mammalian Expression of HsNot1iso1_1318-1605

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1497-1717-G1562E_W

Plasmid

#147873

Purpose

Insect Expression of DmNot1_1497-1717-G1562E

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1497-1717-G1562E (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1497-1717-G1562Y_W

Plasmid

#147874

Purpose

Insect Expression of DmNot1_1497-1717-G1562Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1497-1717-G1562Y (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HsNot1_1318-1605-HA-MBP_W

Plasmid

#147879

Purpose

Mammalian Expression of HsNot1iso1_1318-1605

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-HsNot9shRNA2_W

Plasmid

#147885

Purpose

Mammalian Expression of shRNA2-HsNot9

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

shRNA2-HsNot9 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1467-1717-G1562E_W

Plasmid

#147851

Purpose

Insect Expression of DmNot1_1467-1717-G1562E

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1467-1717-G1562E (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1467-1717-G1562Y_W

Plasmid

#147852

Purpose

Insect Expression of DmNot1_1467-1717-G1562Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1467-1717-G1562Y (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1467-1717-K1537S_W

Plasmid

#147853

Purpose

Insect Expression of DmNot1_1467-1717-K1537S

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1467-1717-K1537S (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1467-1717-K1537SG1562EQ1658A_W

Plasmid

#147854

Purpose

Insect Expression of DmNot1_1467-1717-K1537SG1562EQ1658A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1467-1717-K1537SG1562EQ1658A (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1467-1717-K1569A_W

Plasmid

#147855

Purpose

Insect Expression of DmNot1_1467-1717-K1569A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1467-1717-K1569A (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1467-1717-Q1658A_W

Plasmid

#147856

Purpose

Insect Expression of DmNot1_1467-1717-Q1658A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1467-1717-Q1658A (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmMe31B-Q294AN296F-dsRNAres_W

Plasmid

#147862

Purpose

Insect Expression of DmMe31B-Q294AAN296F-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmMe31B-Q294AAN296F-dsRNAres (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmMe31B-Q294AN296FR347A-dsRNAres_W

Plasmid

#147863

Purpose

Insect Expression of DmMe31B-Q294AAN296FR347A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmMe31B-Q294AAN296FR347A-dsRNAres (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmMe31B-R347A-dsRNAres_W

Plasmid

#147864

Purpose

Insect Expression of DmMe31B-R347A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmMe31B-R347A-dsRNAres (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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