Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

Lentiviral vector for sgRNA expression

Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

mNeonGreen expression with TRE3g promoter

Express NDT1 in S. cerevisiae

Mammalian expression of codon-optimized Artemis.

Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

Inducible shRNA knockdown of both human DDX5 and DDX17 genes

Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

5'UTR stem-loop luciferase reporter: 0kcal/mol

5'UTR stem-loop luciferase reporter: -30kcal/mol

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

For targeted tethering of the MBD domain of human MBD6 using dCas13

Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

mammalian expression of ER localized moxMaple3