We narrowed to 4,471 results for: ARA-2

1. Nsp13 deltaN2-EGFP

   Plasmid

   #165128

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA26_sgRNA-2_M13ps

   Plasmid

   #231412

   Purpose

   M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-EGFP.Flag-EZH2-mt2*

   Plasmid

   #220246

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2* (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS L30 Ruby3-Rab7A

   Plasmid

   #135651

   Purpose

   Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   Ruby3

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.EZH2-mt2

   Plasmid

   #220240

   Purpose

   Expression of an EZH2 mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFl Strep-sumo-TEV-hAgo2 D597N

   Plasmid

   #136236

   Purpose

   Expressing catalytically inactive human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Use

   Tags

   Sterp Sumo

   Expression

   Insect

   Mutation

   D597N

   Promoter

   polyhedrin

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS L30 Flag-Apex-Rab7A

   Plasmid

   #135652

   Purpose

   Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   Apex2 and FLag

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMyc

   Plasmid

   #19400

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Type

   Empty backbone

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHM103

    Plasmid

    #225527

    Purpose

    Encodes constitutive GFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBK.010

    Plasmid

    #187210

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 915-Plakophilin 2a-Flag

    Plasmid

    #32230

    Depositor

    Kathleen Green

    Article

    Chen et al J Biol Chem. 2002 Mar 22;277(12):10512-2

    Insert

    Plakophilin 2a (PKP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3 S6K2 WT

    Plasmid

    #17729

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pFlag-CMV2-WBP2

    Plasmid

    #27478

    Depositor

    Marius Sudol

    Article

    Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

    Insert

    WW domain binding protein-2 (WBP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

15. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

    Plasmid

    #64207

    Purpose

    mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 (CRY2 Mustard Weed)
    RGS4(del 1-33) (RGS4 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAc5.1B-lambdaN-DmPABPC1-siRNAres-V5His6_I

    Plasmid

    #146586

    Purpose

    Insect Expression of DmPABPC1-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

    Insert

    DmPABPC1-siRNAres (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    seven silent mutations compared to the sequence g…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CIPK2-upGFP

    Plasmid

    #117863

    Purpose

    protein expression

    Depositor

    Geoffrey Chang

    Insert

    CIPK2-upGFP (CIPK2 Mustard Weed)
    

    Use

    Tags

    eGFP

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 S6K2 KR

    Plasmid

    #17730

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K99R: kinase dead mutant

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pAc5.1B-EGFP-DmNot2

    Plasmid

    #79255

    Purpose

    Expresses EGFP-tagged DmNot2 in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmNot2 (Rga Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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