We narrowed to 2,039 results for: ARP

1. pcDNA3-DN-hCUL5-FLAG

   Plasmid

   #15823

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin5 (CUL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N441

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-DN-hCUL2-FLAG

   Plasmid

   #15819

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin2 (CUL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N427

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB_TRE3G_scFV-sfGFP

   Plasmid

   #235573

   Purpose

   Dox-inducible expression of control superfolder (sf)GFP fused with scFV

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   superfolder (sf)fGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB_TRE3G_dCas9-5xGCN4_Hygro

   Plasmid

   #235572

   Purpose

   Dox-inducible expression of dCas9-GCN4 & genomic integration

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A; H840A

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEC3135 (pERASE)

   Plasmid

   #220306

   Purpose

   Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTargeT gE

   Plasmid

   #60845

   Purpose

   Expression of the varicella zoster glycoprotein (gE) in mammalian cells

   Depositor

   Charles Grose

   Article

   Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

   Insert

   ORF68 (ORF68 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pHAGE-FLAG-APEX2-GFP

   Plasmid

   #175757

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC2735 (pSpy0C2)

   Plasmid

   #220279

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

   Plasmid

   #214998

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

    Plasmid

    #214999

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE pGK hNLRP3-mNG

    Plasmid

    #218643

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-2TK-PTB

    Plasmid

    #21929

    Depositor

    Phil Sharp

    Article

    Garcia-Blanco et al Genes Dev. 1989 Dec . 3(12A):1874-86.

    Insert

    PTB (PTBP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221 hNLRP3-mNG-STOP

    Plasmid

    #218644

    Purpose

    Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Gateway entry

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC2796 (pSpy0C4)

    Plasmid

    #220280

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

    Plasmid

    #218654

    Purpose

    Knockout vector for mouse Fam160b1 (Fhip2A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Fam160b1 (Fhip2a Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

    Plasmid

    #218640

    Purpose

    Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    delPYD (I125M-END)

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221 hNLRP3 R262W (disease-associated mutation)

    Plasmid

    #218647

    Purpose

    Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    R262W

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 msNLRP3(ΔPYD, I125M-END)

    Plasmid

    #218641

    Purpose

    Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    delPYD (I125M-END)

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

    Plasmid

    #218655

    Purpose

    Knockout vector for mouse Fam160b1 (Fhip2A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Fam160b1 (Fhip2a Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

    Plasmid

    #218636

    Purpose

    Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    H781E, Q782R, F785A

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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