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Plasmid#197355PurposeA knock-out vector for dog ErbB2.DepositorInsertA gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJo55
Plasmid#159623PurposeAn empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Nectin-2
Plasmid#170295PurposeA knock-out vector for the mouse Nectin-2DepositorInsertA gRNA targeting the mouse Nectin-2 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Necl-5
Plasmid#170294PurposeA knock-out vector for the mouse Necl-5DepositorInsertA gRNA targeting the mouse Necl-5 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA40
Plasmid#158518PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA
UseTags6xHAExpressionPlantMutationL17E, D481VPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pZA5
Plasmid#158483PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationSTOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA39
Plasmid#158517PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA
UseTags6xHAExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA30
Plasmid#158508PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
UseTags4xMycExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA32
Plasmid#158510PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
UseTags4xMycExpressionPlantMutationL17E, D481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA24
Plasmid#158502PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E. Km plant selection marker.DepositorInsertZAR1
UseTagsExpressionPlantMutationL17E, D481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA38
Plasmid#158516PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA
UseTags6xHAExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA46
Plasmid#158524PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA47
Plasmid#158525PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA48
Plasmid#158526PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationL17E, D481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFS345
Plasmid#89349PurposeBeetle Luciferase C-term tagging vectorDepositorInsertBeetle luciferase
UseC-terminal tagging vectorTagsExpressionMutationPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbGFP
Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE-tight2-Tmem98-FLAG
Plasmid#124506PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsFlagExpressionMammalianMutationPromotertetO-CMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOsTIR1w/oGFP
Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
UseTags3x mycExpressionYeastMutationPromoterADH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only