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Plasmid#65808Purposeexpression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.DepositorInsertPABPC1 (PABPC2 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationMLLEmutPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
INPP5D-eGFP D673G mutant
Plasmid#161999PurposeMammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)DepositorInsertINPP5D (INPP5D Human)
UseTagseGFPExpressionMammalianMutationD673GPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a] mut
Plasmid#26576DepositorInsertCACNA1D (Cacna1d Rat)
UseTags6XHis and V5ExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-TOPmut-renilla
Plasmid#38236DepositorInsertEef2_TOPm_5UTR/Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationThe first nucleotides of the 5' UTR were cha…PromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
Luc-SIRT1 mutant 3'UTR
Plasmid#20380DepositorInsertSIRT1 (SIRT1 Human)
UseLuciferaseTagsExpressionMutationmutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…PromoterAvailable sinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-Lats1-PY1+2 mutant
Plasmid#19054DepositorInsertLarge tumor suppressor kinase 1, N mutant (LATS1 Human)
UseTagsEGFPExpressionMammalianMutationPY1 mutant, (Tyrosine 376 changed to Alanine) and…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKH143_EGFP-YIPF3 LIR mut
Plasmid#214975PurposeGFP tagged LIR mutant YIPF3DepositorInsertGFP-YIPF3_mut (YIPF3 Human)
UseLentiviralTagsExpressionMutationLIR mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLEX-m-cyclin D1 -T286A mutant
Plasmid#86792Purposemammalian expression of cyclin D1 T286A mutantDepositorInsertcyclin D1 (Ccnd1 Mouse)
UseTagsFLAGExpressionMammalianMutationT286APromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
betaTrCP mVenus mutant reporter
Plasmid#192459PurposeFluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.DepositorInsertCdC25B
UseLentiviralTagsSV40 NLS and mVenusExpressionMammalianMutationaa198-338 of CDC25B. betaTrCP degron sequence (aa…PromoterEF1alphaAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YD EGFP
Plasmid#187264PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsEGFPExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCF176 HLA-A*02:01 (CD8 mut)
Plasmid#180459PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01 (CD8 mut)
UseTagsBSP41ExpressionBacterialMutationD227K, T228A, replacement of transmembrane domain…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 Ets mut
Plasmid#27157PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationR62E/R65E/Y66DPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 mCherry-hCortKD-Flcortmut3YD
Plasmid#187266PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YF mCherry
Plasmid#187265PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YF (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc card9 mutant
Plasmid#16254DepositorInsertcard9 mutant (CARD9 Human)
UseTagsmycExpressionMammalianMutationS528A, T531A, T533A, S536APromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-RAX-MUT
Plasmid#194573PurposeMammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion ProteinDepositorInsertRAX (RAX Human)
UseTagsmEGFPExpressionMammalianMutationS222Rfs*63PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-MYOD1-MUT
Plasmid#194571PurposeMammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion ProteinDepositorInsertMYOD1 (MYOD1 Human)
UseTagsmEGFPExpressionMammalianMutationR188Pfs*90PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PTEN-mut (negative control)
Plasmid#86646Purposeexpression of control shRNADepositorInsertPTEN (control)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EZH2-T487A mutant
Plasmid#28061DepositorInsertEZH2 (EZH2 Human)
UseTagsGSTExpressionBacterialMutationT487APromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only