Addgene

We narrowed to 2,334 results for: crispr system

Showing: 541 - 560 of 2334 results

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pGGB-SpCas9

Plasmid

#210533

Purpose

Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

SpCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ140

Plasmid

#99895

Purpose

Empty vector with attL5-attL2 recombination sites for Gateway Cloning

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x7

Plasmid

#58777

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mCas13 Twin-SUMO

Plasmid

#199753

Purpose

Expresses codon optimized miniature mCas13 for protein expression in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

Insert

mCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

LacI

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2.2

Plasmid

#134752

Purpose

PCR template for sgRNA cloning

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

sgRNA-AtU6_29p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCBC-MT1T2.2

Plasmid

#134751

Purpose

PCR template for sgRNA cloning

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

sgRNA-TaU3p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x4

Plasmid

#58774

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x6

Plasmid

#58776

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmEGFP-C

Plasmid

#113751

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

3xmEGFP

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-N

Plasmid

#113754

Purpose

Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP1

Plasmid

#66581

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pX330.iGFP2

Plasmid

#66582

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pX330.iGFP5

Plasmid

#66584

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pX330.LoxPO

Plasmid

#66585

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mEGFP-C

Plasmid

#113749

Purpose

Gateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mRuby-C

Plasmid

#113755

Purpose

Gateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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