Addgene

We narrowed to 1,635 results for: plasmids pcas

Showing: 541 - 560 of 1635 results

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NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75233

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGmCherry-gRNA

Plasmid

#87110

Purpose

gRNA cloning vector with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-attBr-ZsGreen-attP

Plasmid

#51265

Purpose

Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-ZsGreen-attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xControlgRNA

Plasmid

#224583

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Expression

Mutation

Codon optimized

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ8-CadR-Pcadh-LacZ

Plasmid

#191623

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-eSpCas9(1.1)+K855A

Plasmid

#108298

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-eSpCas9(1.1)+K810A

Plasmid

#108297

Purpose

pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2.12.1 Spike

Plasmid

#186032

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike

Depositor

Marceline Côté

Article

Gong et al Viruses. 2022 Sep 30;14(10):2178. doi: 1

Insert

SARS-CoV-2 BA.2.12.1 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mEos3.2-CCR5TC

Plasmid

#135532

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-eGFP-CCR5TC

Plasmid

#135530

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mNeonGreen-CCR5TC

Plasmid

#135534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-mNeonGreen-CCR5TC

Plasmid

#135533

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-SNAPf-CCR5TC

Plasmid

#135537

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

SNAPf

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-SNAPf-CCR5TC

Plasmid

#135536

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

SNAPf

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph18-eGFP-CCR5TC

Plasmid

#135529

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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